User profiles for Anneliese M.M. Gest
Anneliese MM GestPostdoctoral Scholar, UC San Diego Verified email at health.ucsd.edu Cited by 547 |
Measuring absolute membrane potential across space and time
JR Lazzari-Dean, AMM Gest… - Annual review of …, 2021 - annualreviews.org
Membrane potential (V mem ) is a fundamental biophysical signal present in all cells. V
mem signals range in time from milliseconds to days, and they span lengths from microns to …
mem signals range in time from milliseconds to days, and they span lengths from microns to …
High-throughput evolution of near-infrared serotonin nanosensors
Imaging neuromodulation with synthetic probes is an emerging technology for studying
neurotransmission. However, most synthetic probes are developed through conjugation of …
neurotransmission. However, most synthetic probes are developed through conjugation of …
Optical estimation of absolute membrane potential using fluorescence lifetime imaging
JR Lazzari-Dean, AMM Gest, EW Miller - Elife, 2019 - elifesciences.org
… mM K+ exist as a point of comparison, we obtained a rough estimate of Vmem in 6 mM
extracellular K+ and 120 mM … in 6 mM extracellular K+ and À25 to +2 mV in 120 mM extracellular K…
extracellular K+ and 120 mM … in 6 mM extracellular K+ and À25 to +2 mV in 120 mM extracellular K…
Molecular prosthetics for long-term functional imaging with fluorescent reporters
Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential
in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the …
in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the …
Bioorthogonal, fluorogenic targeting of voltage-sensitive fluorophores for visualizing membrane potential dynamics in cellular organelles
Electrical potential differences across lipid bilayers play foundational roles in cellular
physiology. Plasma membrane voltage is the most widely studied; however, the bilayers of …
physiology. Plasma membrane voltage is the most widely studied; however, the bilayers of …
A red-emitting carborhodamine for monitoring and measuring membrane potential
AMM Gest, JR Lazzari-Dean, G Ortiz… - Proceedings of the …, 2024 - National Acad Sciences
Biological membrane potentials, or voltages, are a central facet of cellular life. Optical
methods to visualize cellular membrane voltages with fluorescent indicators are an attractive …
methods to visualize cellular membrane voltages with fluorescent indicators are an attractive …
Optical Estimation of Membrane Potential Values Using Fluorescence Lifetime Imaging Microscopy and Hybrid Chemical-Genetic Voltage Indicators
Introduction: Membrane potential (V m ), the voltage across a cell membrane, is an important
biophysical phenomenon, central to the physiology of cells, tissues, and organisms. Voltage…
biophysical phenomenon, central to the physiology of cells, tissues, and organisms. Voltage…
VoltageFluor dyes and fluorescence lifetime imaging for optical measurement of membrane potential
AMM Gest, SK Yaeger-Weiss, JR Lazzari-Dean… - Methods in …, 2021 - Elsevier
Membrane potential is a fundamental biophysical parameter common to all of cellular life.
Traditional methods to measure membrane potential rely on electrodes, which are invasive …
Traditional methods to measure membrane potential rely on electrodes, which are invasive …
[HTML][HTML] A colorimetric competitive displacement assay for the evaluation of catalytic peptides
An indicator displacement assay has been adapted to detect the diol products of the aldol
reaction between 4-nitrobenzaldehyde and hydroxyacetone in crude reaction mixtures. This …
reaction between 4-nitrobenzaldehyde and hydroxyacetone in crude reaction mixtures. This …
The Taf14 YEATS domain is a reader of histone crotonylation
… Cells were harvested by centrifugation and resuspended in 50 mM HEPES (pH 7.5)
supplemented with 150 mM NaCl and 1 mM TCEP. Cells are lysed by freeze–thaw followed by …
supplemented with 150 mM NaCl and 1 mM TCEP. Cells are lysed by freeze–thaw followed by …