A shuttle vector designated pMAD was constructed for quickly generating gene inactivation
mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-…

The peptidoglycan cell wall and the actin-like MreB cytoskeleton are major determinants of
cell shape in rod-shaped bacteria. The prevailing model postulates that helical, membrane-…

The Hsp100/Clp ATPases constitute a family of closely related proteins of which some
members function solely as chaperones whereas others additionally can associate with the …

In vitro mariner transposon mutagenesis ofStreptococcus pneumoniae chromosomal DNA
was used to isolate regulatory mutants affecting expression of thecomCDE operon, encoding …

A model system for investigating how developmental regulatory networks determine cell fate
is spore formation in Bacillus subtilis. The master regulator for sporulation is Spo0A, which …

Multiple regulatory mechanisms for coping with stress co‐exist in low G+C Gram‐positive
bacteria. Among these, the HrcA and CtsR repressors control distinct regulons in the model …

Clp-HSP100 ATPases are a widespread family of ubiquitous proteins that occur in both
prokaryotes and eukaryotes and play important roles in the folding of newly synthesized proteins …

The AbrB protein of the spore-forming bacterium Bacillus subtilis is a repressor of numerous
genes that are switched on during the transition from the exponential to the stationary phase …

How cells control their shape and size is a long-standing question in cell biology. Many rod-shaped
bacteria elongate their sidewalls by the action of cell wall synthesizing machineries …

The cytoskeleton occupies a central role in cellular immunity by promoting bacterial sensing
and antibacterial functions. Septins are cytoskeletal proteins implicated in various cellular …