The human pathogen Pseudomonas aeruginosa produces pyocyanin, a blue-pigmented
phenazine derivative, which is known to play a role in virulence. Pyocyanin is produced from …

The design of proteins that bind to a specific site on the surface of a target protein using no
information other than the three-dimensional structure of the target remains a challenge 1 , 2 , …

There has been considerable recent progress in protein structure prediction using deep neural
networks to predict inter-residue distances from amino acid sequences 1 , 2 – 3 . Here we …

These findings show that oncogenic KRAS Q61H forms a cooperative RAS–RAF ternary
complex, which renders RAS-driven tumors vulnerable to MEKi and RAFi, thus establishing a …

INTRODUCTION Despite their key biological roles, only a few proteins that fold into lipid
membranes have been designed de novo. A class of membrane proteins—transmembrane β …

Although epidemiologic and clinical studies have suggested allele-specific behaviors for
KRAS , experimental evidence for allele-specific biological properties is limited. We combined …

Although deep learning has revolutionized protein structure prediction, almost all experimentally
characterized de novo protein designs have been generated using physically based …

Deep learning methods have revolutionized protein structure prediction and design but are
currently limited to protein-only systems. We describe RoseTTAFold All-Atom (RFAA) which …

We use computational design coupled with experimental characterization to systematically
investigate the design principles for macrocycle membrane permeability and oral …

Tumor necrosis factor alpha (TNF-α) has both positive and negative roles in human disease.
In certain cancers, TNF-α is infused locally to promote tumor regression, but dose-limiting …