Vitamin C is an antioxidant that maintains the activity of iron and α‐ketoglutarate‐dependent
dioxygenases. Despite these enzymes being implicated in a wide range of biological …

In mammals, the noncoding Xist RNA triggers transcriptional silencing of one of the two X
chromosomes in female cells. Here, we report a genetic screen for silencing factors in X …

Full thickness wounds require a dermal component to achieve functional permanent skin
restoration. Currently available tissue‐engineered skin substitutes lack a subcutaneous fat …

Female mammals express the long noncoding X inactivation-specific transcript (Xist) RNA
to initiate X chromosome inactivation (XCI) that eventually results in the formation of the Barr …

Hedgehog (HH) signaling is important for embryonic pattering and stem cell differentiation.
The G protein–coupled receptor (GPCR) Smoothened (SMO) is the key HH signal transducer …

The Xist gene produces a long noncoding RNA that initiates chromosome-wide gene repression
on the inactive X chromosome in female mammals. Recent progress has advanced the …

Mouse parthenogenetic haploid embryonic stem cells (ESCs) are pluripotent cells generated
from chemically activated oocytes. Haploid ESCs provide an opportunity to study the effect …

CRISPR-associated (Cas) nucleases are established tools for engineering of animal genomes.
These programmable RNA-guided nucleases have been introduced into zygotes using …

CRISPR–Cas12a is a bacterial RNA-guided deoxyribonuclease that has been adopted for
genetic engineering in a broad variety of organisms. Here, we describe protocols for the …

In plastic and reconstructive surgery there is an increasing demand for malleable implants
to repair soft tissue congenital defects, or those resulting from aging, traumatic injury and …