Skeletal muscle mass, function, and repair capacity all progressively decline with aging,
restricting mobility, voluntary function, and quality of life. Skeletal muscle repair is facilitated by a …

Satellite cells are skeletal muscle stem cells with a principal role in postnatal skeletal muscle
regeneration. Satellite cells, like many tissue-specific adult stem cells, reside in a quiescent …

… -μm and 40-μm filters (BD Biosciences) to generate single-cell suspensions. We first incubated
cells with biotinylated antibodies reactive to CD45 (BD Biosciences, clone 30F11, catalog …

During mesenchymal development, the microenvironment gradually transitions from one
that is rich in cell–cell interactions to one that is dominated by cell–ECM (extracellular matrix) …

Mechanical forces transduced to cells through the extracellular matrix are critical regulators
of tissue development, growth, and homeostasis, and can play important roles in directing …

… The authors acknowledge technical assistance from Hannah Fong of the Cosgrove group
… The authors are also thankful for the constructive discussions with members of the Cosgrove…

Skeletal muscle repair is driven by the coordinated self-renewal and fusion of myogenic
stem and progenitor cells. Single-cell gene expression analyses of myogenesis have been …

Tissue fibrosis contributes to nearly half of all deaths in the developed world and is
characterized by progressive matrix stiffening. Despite this, nearly all in vitro disease models are …

… set or BD Phosflow fix buffer (BD, catalogue number 557870) when antigen phosphorylation
was of interest. After fixation, cells were incubated in permeabilization buffer with antibodies …

Spatial transcriptomics reveals the spatial context of gene expression, but current methods
are limited to assaying polyadenylated (A-tailed) RNA transcripts. Here we demonstrate that …