Many neurodegenerative diseases, including Alzheimer's, originate from the conversion of
proteins into pathogenic conformations. The microtubule-associated protein tau converts into …

… A BD LSRFortessa was used to perform FRET flow cytometry. To measure CFP and FRET,
cells were excited with the 405 nm laser, and fluorescence was captured with a 405/50 nm …

Cryogenic electron microscopy has revealed unprecedented molecular insight into the
conformations of β-sheet-rich protein amyloids linked to neurodegenerative diseases. It remains …

… A BD LSRFortessa was used to perform FRET flow cytometry. To measure mCerulean and
FRET signal, cells were excited with the 405 nm laser and fluorescence was captured with a …

The Hsp40/Hsp70 chaperone families combine versatile folding capacity with high substrate
specificity, which is mainly facilitated by Hsp40s. The structure and function of many Hsp40s …

… A BD LSRFortessa SORP was used to perform FRET flow cytometry. To measure mCerulean
and FRET signal, cells were excited with the 405-nm laser and fluorescence was captured …

… BD Ryder and LA Joachimiak analyzed crosslinking data and performed Rosetta simulations.
N. Familiari performed baculovirus-mediated protein expression and immunofluorescence …

J-domain protein (JDP) molecular chaperones have emerged as central players that
maintain a healthy proteome. The diverse members of the JDP family function as monomers/dimers …

Protein quality control (PQC) is carried out in part by the chaperone Hsp70, in concert with
adapters of the J-domain protein (JDP) family. The JDPs, also called Hsp40s, are thought to …

Centrosomes organize microtubules for mitotic spindle assembly and positioning. Forces
mediated by these microtubules create tensile stresses on pericentriolar material (PCM), the …