User profiles for B. Liffner

Benjamin Liffner

Indiana University School of Medicine
Verified email at iu.edu
Cited by 278

[HTML][HTML] The dynamic roles of the inner membrane complex in the multiple stages of the malaria parasite

JL Ferreira, D Heincke, JS Wichers, B Liffner… - Frontiers in Cellular …, 2021 - frontiersin.org
Apicomplexan parasites, such as human malaria parasites, have complex lifecycles encompassing
multiple and diverse environmental niches. Invading, replicating, and escaping from …

Expansion microscopy of apicomplexan parasites

B Liffner, S Absalon - Molecular Microbiology, 2023 - Wiley Online Library
… for harvesting malaria parasites at multiple timepoints/ lifecycle stages (Liffner & Absalon,
2021), along with the testing of a wide number of marker antibodies and chemical dyes (Liffner

Atlas of Plasmodium falciparum intraerythrocytic development using expansion microscopy

B Liffner, AKC Diaz, J Blauwkamp, D Anaguano… - Elife, 2023 - elifesciences.org
… (b) U- ExM workflow used in this study. PFA = paraformaldehyde, FA = formaldehyde, AA =
acrylamide, PG = propyl gallate. Snowflake indicates steps where gels were cryopreserved. …

[HTML][HTML] Expansion Microscopy Reveals Plasmodium falciparum Blood-Stage Parasites Undergo Anaphase with A Chromatin Bridge in the Absence of Mini …

B Liffner, S Absalon - Microorganisms, 2021 - mdpi.com
… Given that each of the microtubule structures could be distinguished from each other
using U-ExM (Figure 1b), we used this technique to study microtubule formation in MCMBP-…

Dissecting the gene expression, localization, membrane topology, and function of the Plasmodium falciparum STEVOR protein family

…, J Strauss, S Witt, A Lill, LI Ehnold, N Neupert, B Liffner… - MBio, 2019 - Am Soc Microbiol
… (B) Line plot showing smoothed conditional means of log 2 FPKM stevor gene expression
values as calculated with the LOESS smoothing method, including their 0.95 confidence …

[HTML][HTML] PfCERLI1 is a conserved rhoptry associated protein essential for Plasmodium falciparum merozoite invasion of erythrocytes

B Liffner, S Frölich, GK Heinemann, B Liu… - Nature …, 2020 - nature.com
b The amino acid sequence of PfCERLI1 was compared against Plasmodium spp. … that
amplify only WT Pfcerli1 locus (primer A and B) or primers that amplify only integrated Pfcerli1 …

The ins and outs of Plasmodium rhoptries, focusing on the cytosolic side

B Liffner, JM Balbin, JS Wichers, TW Gilberger… - Trends in …, 2021 - cell.com
Parasites of the genus Plasmodium cause human and animal malaria, leading to significant
health and economic impacts. A key aspect of the complex life cycle of Plasmodium …

[PDF][PDF] Structure-based identification and functional characterization of a lipocalin in the malaria parasite Plasmodium falciparum

…, K Lauk, A Zurborg, C Soehnchen, B Liffner… - Cell Reports, 2020 - cell.com
… The overall monomeric structure of PfLCN reveals the classic lipocalin fold with a b-barrel
calyx core consisting of eight hydrogen-bonded anti-parallel b strands and an N-terminal a he…

[HTML][HTML] Retargeting azithromycin analogues to have dual-modality antimalarial activity

…, G Siddiqui, AE De Paoli, D Anderson, B Liffner… - BMC biology, 2020 - Springer
b Schematic of drug washout treatment scheme to assess azithromycin’s quick-killing
stage of activity. Early ring-stage parasites (0–4 hrs post-invasion) were aliquoted to a 96-well …

Structural insights into PfARO and characterization of its interaction with PfAIP

…, J Strauss, A Lill, R Thuenauer, B Liffner… - Journal of molecular …, 2020 - Elsevier
… Arrows (P1–P4) are indicating position of oligonucleotides used for B. (B) PCR analysis of
the rendered genomic locus using primers as shown in the schema using gDNA of 3D7 wild …