The mammalian lung is a highly branched network in which the distal regions of the bronchial
tree transform during development into a densely packed honeycomb of alveolar air sacs …

The human brain has undergone substantial change since humans diverged from chimpanzees
and the other great apes 1 , 2 . However, the genetic and developmental programs that …

Interest in single-cell whole-transcriptome analysis is growing rapidly, especially for profiling
rare or heterogeneous populations of cells. We compared commercially available single-…

Direct lineage reprogramming represents a remarkable conversion of cellular and
transcriptome states 1 , 2 , 3 . However, the intermediate stages through which individual cells …

The Human Developmental Cell Atlas (HDCA) initiative, which is part of the Human Cell Atlas,
aims to create a comprehensive reference map of cells during development. This will be …

Organs are composed of diverse cell types that traverse transient states during organogenesis.
To interrogate this diversity during human development, we generate a single-cell …

Broadly, tissue regeneration is achieved in two ways: by proliferation of common differentiated
cells and/or by deployment of specialized stem/progenitor cells. Which of these pathways …

Conventional two-dimensional differentiation from pluripotency fails to recapitulate cell
interactions occurring during organogenesis. Three-dimensional organoids generate complex …

Cerebral organoids—3D cultures of human cerebral tissue derived from pluripotent stem
cells—have emerged as models of human cortical development. However, the extent to which …

The striatum contributes to many cognitive processes and disorders, but its cell types are
incompletely characterized. We show that microfluidic and FACS-based single-cell RNA …