One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

KA Datsenko, BL Wanner - Proceedings of the National …, 2000 - National Acad Sciences
We have developed a simple and highly efficient method to disrupt chromosomal genes in
Escherichia coli in which PCR primers provide the homology to the targeted gene(s). In this …

Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection

…, KA Datsenko, M Tomita, BL Wanner… - Molecular systems …, 2006 - embopress.org
We have systematically made a set of precisely defined, single‐gene deletions of all
nonessential genes in Escherichia coli K‐12. Open‐reading frame coding regions were replaced …

Minimum information requested in the annotation of biochemical models (MIRIAM)

…, B Shapiro, JL Snoep, HD Spence, BL Wanner - Nature …, 2005 - nature.com
Most of the published quantitative models in biology are lost for the community because
they are either not made available or they are insufficiently characterized to allow them to be …

Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria

A Haldimann, BL Wanner - Journal of bacteriology, 2001 - Am Soc Microbiol
We have developed a series of powerful and versatile conditional-replication, integration,
and modular (CRIM) plasmids. CRIM plasmids can be replicated at medium or high copy …

Escherichia coli K-12: a cooperatively developed annotation snapshot—2005

…, NR Thomson, D Wishart, BL Wanner - Nucleic acids …, 2006 - academic.oup.com
The goal of this group project has been to coordinate and bring up-to-date information on all
genes of Escherichia coli K-12. Annotation of the genome of an organism entails …

[HTML][HTML] Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system

…, K Pougach, A Tikhonov, BL Wanner… - Nature …, 2012 - nature.com
CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated
genes) is a small RNA-based adaptive prokaryotic immunity system that functions by …

Highly accurate genome sequences of Escherichia coli K‐12 strains MG1655 and W3110

…, S Choi, E Ohtsubo, T Baba, BL Wanner… - Molecular systems …, 2006 - embopress.org
With the goal of solving the whole‐cell problem with Escherichia coli K‐12 as a model cell,
highly accurate genomes were determined for two closely related K‐12 strains, MG1655 and …

[PDF][PDF] Phosphorus assimilation and control of the phosphate regulon

BL Wanner - Escherichia coli and Salmonella: cellular and …, 1996 - academia.edu
This chapter concerns the genes and gene products belonging to the phosphate (Pho) regulon
of Escherichia coli and the regulation of these genes. By definition, all genes of a regulon …

Conditionally replicative and conjugative plasmids CarryinglacZα for cloning, mutagenesis, and allele replacement in bacteria

…, LL Daniels, SK Kim, A Haldimann, BL Wanner - Plasmid, 1996 - Elsevier
We describe several new cloning vectors for mutagenesis and allele replacement experiments.
These plasmids have the R6Kγ DNA replication origin (oriR R6Kγ ) so they replicate only …

The phosphate regulon and bacterial virulence: a regulatory network connecting phosphate homeostasis and pathogenesis

MG Lamarche, BL Wanner, S Crépin… - FEMS microbiology …, 2008 - academic.oup.com
Bacterial pathogens regulate virulence factor gene expression coordinately in response to
environmental stimuli, including nutrient starvation. The phosphate (Pho) regulon plays a key …