The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription
factor. However, modest levels of gene activation have limited potential applications. We …

Several programmable transcription factors exist based on the versatile Cas9 protein, yet
their relative potency and effectiveness across various cell types and species remain …

The ability to engineer genomes in a specific, systematic, and cost-effective way is critical
for functional genomic studies. Recent advances using the CRISPR-associated single-guide …

10.7554/eLife.16582.001 How proteins control the biogenesis of cellular lipid droplets (LDs)
is poorly understood. Using Drosophila and human cells, we show here that seipin, an ER …

10.7554/eLife.35574.001 We generated a library of ~1000 Drosophila stocks in which we
inserted a construct in the intron of genes allowing expression of GAL4 under control of …

Our understanding of the genetic mechanisms that underlie biological processes has relied
extensively on loss-of-function (LOF) analyses. LOF methods target DNA, RNA or protein to …

A number of approaches for Cas9-mediated transcriptional activation have recently been
developed, allowing target genes to be overexpressed from their endogenous genomic loci. …

The mechanistic target of rapamycin complex 1 (mTORC1) supports proliferation through
parallel induction of key anabolic processes, including protein, lipid, and nucleotide synthesis. …

The tuberous sclerosis complex (TSC) family of tumor suppressors, TSC1 and TSC2, function
together in an evolutionarily conserved protein complex that is a point of convergence for …

The rapid rise of CRISPR as a technology for genome engineering and related research
applications has created a need for algorithms and associated online tools that facilitate design …