Genetic and biochemical analyses of the secretory pathway have produced a detailed picture
of the molecular mechanisms involved in selective cargo transport between organelles. …

The red fluorescent protein DsRed has spectral properties that are ideal for dual-color
experiments with green fluorescent protein (GFP) 1 . But wild-type DsRed has several drawbacks, …

N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the
Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, …

Isolated Golgi membranes incubated in the presence of ATP and a cytosolic protein fraction
form a population of coated buds or vesicles from the Golgi cisternae. The coats do not have …

The pathway by which cytochromes cl and b2 reach the mitochondrial intermembrane space
has been controversial. According to the “conservative sorting” hy pothesis, these proteins …

The Golgi apparatus is composed of biochemically distinct early (cis, medial) and late (trans,
TGN) cisternae. There is debate about the nature of these cisternae 1 , 2 , 3 . The stable …

An N-ethylmaleimide-sensitive transport component (NSF) has been purified on the basis of
its ability to support transport between Golgi cisternae. We now report that NSF is needed …

Golgi stacks are often located near sites of “transitional ER” (tER), where COPII transport
vesicles are produced. This juxtaposition may indicate that Golgi cisternae form at tER sites. To …

Newly synthesized secretory cargo molecules pass through the Golgi apparatus while resident
Golgi proteins remain in the organelle. However, the pathways of membrane traffic within …

Publisher Summary This chapter focuses on the isolation of highly purified mitochondria
from Saccharomyces cerevisiae and the purification of mitochondria from a 10-liter culture of …