Resolution in optical nanoscopy (or super-resolution microscopy) depends on the localization
uncertainty and density of single fluorescent labels and on the sample's spatial structure. …

A study of the uncertainty of localizing single‐molecule emitters for super‐resolution light
microscopy is presented. Maximum likelihood estimation (MLE) is found to be superior to least‐…

We describe an iterative algorithm that converges to the maximum likelihood estimate of the
position and intensity of a single fluorophore. Our technique efficiently computes and …

Current methods for detector gain calibration require acquisition of tens of special calibration
images. Here we propose a method that obtains the gain from the actual image for which …

In microscopy, single fluorescence point sources can be localized with a precision several
times greater than the resolution limit of the microscope. We show that the intermittent …

One of the most complex molecular machines of cells is the nuclear pore complex (NPC),
which controls all trafficking of molecules in and out of the nucleus. Because of their …

With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-molecule
localization microscopy (SMLM), a large set of different data analysis packages have …

ErbB1 receptors situated on cellular filopodia undergo systematic retrograde transport after
binding of the epidermal growth factor (EGF) and activation of the receptor tyrosine kinase. …

The Gaussian function is simple and easy to implement as Point Spread Function (PSF)
model for fitting the position of fluorescent emitters in localization microscopy. Despite its …

The wormlike chain model describes the micromechanics of semiflexible polymers by
introducing the persistence length. We propose a method of measuring the persistence length of …