Many industrial chemicals that are produced from fossil resources could be manufactured
more sustainably through fermentation. Here we describe the development of a carbon-…

Owing to rising levels of greenhouse gases in our atmosphere and oceans, climate change
poses significant environmental, economic, and social challenges globally. Technologies …

The design and optimization of biosynthetic pathways for industrially relevant, non-model
organisms is challenging due to transformation idiosyncrasies, reduced numbers of validated …

Highlights • Cell-free systems enable biochemical reactions in the absence of cell viability
constraints. • Cell-free metabolic engineering presents opportunities for biomanufacturing …

Streptomyces have a rich history as producers of important natural products and this genus
of bacteria has recently garnered attention for its potential applications in the broader context …

Styrene is an important petroleum-derived molecule that is polymerized to make versatile
plastics, including disposable silverware and foamed packaging materials. Finding more …

Cell-free systems are useful tools for prototyping metabolic pathways and optimizing the
production of various bioproducts. Mechanistically-based kinetic models are uniquely suited to …

Cell-free systems using crude cell extracts present appealing opportunities for designing
biosynthetic pathways and enabling sustainable chemical synthesis. However, the lack of tools …

Cell-free systems derived from crude cell extracts have developed into tools for gene
expression, with applications in prototyping, biosensing, and protein production. Key to the …

Cell-free synthetic biology is a maturing field that aims to assemble biomolecular reactions
outside cells for compelling applications in drug discovery, metabolic engineering, …