User profiles for Bo-Jui Chang

Bo-Jui Chang

UT Southwestern Medical center
Verified email at utsouthwestern.edu
Cited by 1319

Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution

…, E Jeffery, MM Murphy, P Roudot, BJ Chang… - Nature …, 2019 - nature.com
We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables
isotropic, subcellular resolution imaging with high optical sectioning capability and a large field …

Universal light-sheet generation with field synthesis

BJ Chang, M Kittisopikul, KM Dean, P Roudot… - Nature …, 2019 - nature.com
We introduce field synthesis, a theorem and method that can be used to synthesize any
scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), …

[PDF][PDF] A single-objective light-sheet microscope with 200 nm-scale resolution

E Sapoznik, BJ Chang, RJ Ju, ES Welf, D Broadbent… - BioRxiv, 2020 - academia.edu
We present a single-objective light-sheet microscope, also known as an oblique-plane
microscope, that uses a bespoke glass-tipped tertiary objective and improves the resolution, field …

Light sheet fluorescence microscopy

EHK Stelzer, F Strobl, BJ Chang, F Preusser… - Nature Reviews …, 2021 - nature.com
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only fluorophores
within the focal volume. Light sheet microscopes (LSMs) have a true optical sectioning …

A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics

E Sapoznik, BJ Chang, J Huh, RJ Ju, EV Azarova… - Elife, 2020 - elifesciences.org
We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary
objective to improve the resolution, field of view, and usability over previous variants. Owing to …

Resolution doubling in light-sheet microscopy via oblique plane structured illumination

B Chen, BJ Chang, P Roudot, F Zhou, E Sapoznik… - Nature …, 2022 - nature.com
Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence
microscope without requiring high laser powers or specialized fluorophores. However, the …

Light sheet-based fluorescence microscopy (LSFM) for the quantitative imaging of cells and tissues

F Pampaloni, BJ Chang, EHK Stelzer - Cell and tissue research, 2015 - Springer
In light sheet-based fluorescence microscopy (LSFM), only the focal plane is illuminated by
a laser light sheet. Hence, only the fluorophores within a thin volume of the specimen are …

Isotropic image in structured illumination microscopy patterned with a spatial light modulator

BJ Chang, LJ Chou, YC Chang, SY Chiang - Optics express, 2009 - opg.optica.org
We developed a structured illumination microscopy (SIM) system that uses a spatial light
modulator (SLM) to generate interference illumination patterns at four orientations – 0, 45, 90, …

Blebs promote cell survival by assembling oncogenic signalling hubs

…, MK Driscoll, FY Zhou, H Mazloom-Farsibaf, BJ Chang… - Nature, 2023 - nature.com
Most human cells require anchorage for survival. Cell–substrate adhesion activates diverse
signalling pathways, without which cells undergo anoikis—a form of programmed cell death …

csiLSFM combines light-sheet fluorescence microscopy and coherent structured illumination for a lateral resolution below 100 nm

BJ Chang, VD Perez Meza… - Proceedings of the …, 2017 - National Acad Sciences
Light-sheet-based fluorescence microscopy (LSFM) features optical sectioning in the excitation
process. It minimizes fluorophore bleaching as well as phototoxic effects and provides a …