Replication intermediates from the yeast 2pm plasmid and a recombinant plasmid containing
the yeast autonomous replication sequence ARSl have been analyzed by twedimensional …

Oligonucleotide microarrays were used to map the detailed topography of chromosome
replication in the budding yeastSaccharomyces cerevisiae. The times of replication of thousands …

… Herr (Cold Spring Harbor, USA) J. Hodgkin (Cambridge, UK) R. Horvitz (Cambridge, USA)
P. … J. Cuddihy (Cold Spring Harbor) … Brewer, and Walton L. Fangman …

Replication of the-200 tandem copies of yeast ribosomal RNA genes (I-DNA) is known to be
initiated within a subset of the repeats, with transcription continuing during the replication …

The temporal firing of replication origins throughout S phase in yeast depends on unknown
determinants within the adjacent chromosomal environment. We demonstrate here that the …

Replication forks, moving opposite to the direction of transcription, are arrested at the 3’ends
of the 3% transcription units in the rDNA locus of S. cerevisiae. Because of its position and …

Hydroxyurea (HU) is a DNA replication inhibitor that negatively affects both the elongation
and initiation phases of replication and triggers the “intra-S phase checkpoint.” Previous work …

Initiation of eukaryotic DNA replication requires phosphorylation of the MCM complex by
Dbf4-dependent kinase (DDK), composed of Cdc7 kinase and its activator, Dbf4. We report …

Publisher Summary This chapter focuses on the analysis of replication intermediates by two-dimensional
(2D) agarose gel electrophoresis. The first step in the analysis of replication …

During DNA replication one or both strands transiently become single stranded: first at the
sites where initiation of DNA synthesis occurs (known as origins of replication) and …