Forward genetic screens in model organisms are vital for identifying novel genes essential
for developmental or disease processes. One drawback of these screens is the labor-…

Background: Genome editing techniques, including ZFN, TALEN, and CRISPR, have created
a need to rapidly screen many F1 individuals to identify carriers of indels and determine …

The genetic architecture of sporadic congenital heart disease (CHD) is characterized by
enrichment in damaging de novo variants in chromatin-modifying genes. To test the hypothesis …

Human amniotic fluid contains cells that potentially have important stem cell characteristics,
yet the programs controlling their developmental potency are unclear. Here, we provide …

Neural crest cells migrate to the embryonic heart and transform into a small number of
cardiomyocytes, but their functions in the developing and adult heart are unknown. Here, we show …

Kabuki Syndrome patients have a spectrum of congenital disorders, including congenital
heart defects, the primary determinant of mortality. Seventy percent of Kabuki Syndrome …

Congenital diaphragmatic hernia (CDH) is a developmental defect of the diaphragm that
causes high newborn mortality. Isolated or non‐syndromic CDH is considered a multifactorial …

Current methods to isolate rare (1:10,000–1:100,000) bacterial artificial chromosome (BAC)
recombinants require selectable markers. For seamless BAC mutagenesis, selectable …

We report that lack of crossover along one chromosome arm is associated with high-frequency
occurrence of recombination close to the opposing arm's centromere during zebrafish …

Syndecan 2 (Sdc2) is a transmembrane cell-surface heparan sulfate proteoglycan (HSPG)
that has been implicated in the regulation of cell-cell signaling pathways and cell-matrix …