[HTML][HTML] Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics
BL Scott, KA Sochacki, ST Low-Nam, EM Bailey… - Nature …, 2018 - nature.com
Clathrin-mediated endocytosis (CME) internalizes plasma membrane by reshaping small
regions of the cell surface into spherical vesicles. The key mechanistic question of how coat …
regions of the cell surface into spherical vesicles. The key mechanistic question of how coat …
[HTML][HTML] The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy
Macropinosomes are formed by shaping actin-rich plasma membrane ruffles into large
intracellular organelles in a phosphatidylinositol 3-kinase (PI3K)-coordinated manner. Here, we …
intracellular organelles in a phosphatidylinositol 3-kinase (PI3K)-coordinated manner. Here, we …
[HTML][HTML] N-way FRET microscopy of multiple protein-protein interactions in live cells
AD Hoppe, BL Scott, TP Welliver, SW Straight… - PloS one, 2013 - journals.plos.org
Fluorescence Resonance Energy Transfer (FRET) microscopy has emerged as a powerful
tool to visualize nanoscale protein-protein interactions while capturing their microscale …
tool to visualize nanoscale protein-protein interactions while capturing their microscale …
[PDF][PDF] Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis
RH Anderson, KA Sochacki, H Vuppula, BL Scott… - Cell reports, 2021 - cell.com
Clathrin-mediated endocytosis (CME) is critical for cellular signal transduction, receptor
recycling, and membrane homeostasis in mammalian cells. Acute depletion of cholesterol …
recycling, and membrane homeostasis in mammalian cells. Acute depletion of cholesterol …
[HTML][HTML] TIRF imaging of Fc gamma receptor microclusters dynamics and signaling on macrophages during frustrated phagocytosis
J Lin, S Kurilova, BL Scott, E Bosworth, BE Iverson… - BMC immunology, 2016 - Springer
Background Recent evidence indicates that in addition to the T-cell receptor, microclustering
is an important mechanism for the activation of the B-cell receptor and the mast cell Fcε-…
is an important mechanism for the activation of the B-cell receptor and the mast cell Fcε-…
[HTML][HTML] Optimizing fluorescent protein trios for 3-Way FRET imaging of protein interactions in living cells
BL Scott, AD Hoppe - Scientific reports, 2015 - nature.com
Powerful new methods have extended FRET microscopy to the imaging of three or more
interacting proteins inside living cells. Here, we compared widely available fluorescent proteins …
interacting proteins inside living cells. Here, we compared widely available fluorescent proteins …
Dynamic raman scattering studies of coated gold nanoparticles: 4-mercaptopyridine, 4-mercaptophenol, and benzenethiol
BL Scott, KT Carron - The Journal of Physical Chemistry C, 2016 - ACS Publications
We have demonstrated several benefits of dynamic Raman scattering (DRS) measurements
on coated gold nanoparticles. DRS examines particles moving in and out of a focused laser …
on coated gold nanoparticles. DRS examines particles moving in and out of a focused laser …
FRETting about the affinity of bimolecular protein–protein interactions
Fluorescence resonance energy transfer (FRET) is a powerful tool to study macromolecular
interactions such as protein–protein interactions (PPIs). Fluorescent protein (FP) fusions …
interactions such as protein–protein interactions (PPIs). Fluorescent protein (FP) fusions …
[HTML][HTML] Three-dimensional reconstruction of three-way fret microscopy improves imaging of multiple protein-protein interactions
BL Scott, AD Hoppe - PLoS One, 2016 - journals.plos.org
Fluorescence resonance energy transfer (FRET) microscopy is a powerful tool for imaging
the interactions between fluorescently tagged proteins in two-dimensions. For FRET …
the interactions between fluorescently tagged proteins in two-dimensions. For FRET …
Antibody surface mobility amplifies FcγR signaling via Arp2/3 during phagocytosis
…, JG Kerkvliet, EM Bailey, RB Anderson, BL Scott… - Biophysical …, 2024 - cell.com
We report herein that the anti-CD20 therapeutic antibody, rituximab, is rearranged into
microclusters within the phagocytic synapse by macrophage Fcγ receptors (FcγR) during antibody…
microclusters within the phagocytic synapse by macrophage Fcγ receptors (FcγR) during antibody…