User profiles for Brenda Oppert

Brenda Oppert

USDA ARS Center for Grain and Animal Health Research
Verified email at ars.usda.gov
Cited by 7726

[HTML][HTML] Proteinase-mediated insect resistance to Bacillus thuringiensis toxins

B Oppert, KJ Kramer, RW Beeman, D Johnson… - Journal of Biological …, 1997 - ASBMB
Two Bacillus thuringiensis(Bt)-resistant strains of the Indianmeal moth, Plodia interpunctella,
lack a major gut proteinase that activates Bt protoxins. The absence of this enzyme is …

[PDF][PDF] The genome of the model beetle and pest Tribolium castaneum.

…, CS White, R Wright, Y Park, J Lord, B Oppert… - Nature, 2008 - serval.unil.ch
Tribolium castaneum is a member of the most species-rich eukaryotic order, a powerful model
organism for the study of generalized insect development, and an important pest of stored …

[HTML][HTML] A model species for agricultural pest genomics: the genome of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

…, M Munoz-Torres, A Muszewska, B Oppert… - Scientific reports, 2018 - nature.com
The Colorado potato beetle is one of the most challenging agricultural pests to manage. It
has shown a spectacular ability to adapt to a variety of solanaceaeous plants and variable …

Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge

JC Lord, K Hartzer, M Toutges, B Oppert - Journal of microbiological …, 2010 - Elsevier
To investigate gene expression in Tribolium castaneum exposed to Beauveria bassiana,
reference genes for qPCR were evaluated. Of these, β-actin, α-tubulin, and RPS6 were not …

Protease interactions with Bacillus thuringiensis insecticidal toxins

B Oppert - Archives of Insect Biochemistry and Physiology …, 1999 - Wiley Online Library
The microbe Bacillus thuringiensis (Bt) produces crystals that contain insecticidal crystal
proteins (ICPs) used to control many major pests. ICPs are degraded by proteases from a …

[HTML][HTML] Insect resistance to Bacillus thuringiensis: alterations in the indianmeal moth larval gut proteome

M Candas, O Loseva, B Oppert, P Kosaraju… - Molecular & Cellular …, 2003 - ASBMB
Insect resistance to the Cry toxins of Bacillus thuringiensis (Bt) has been examined previously
using a number of traditional biochemical and molecular techniques. In this study, we …

Comparative analysis of proteinase activities of Bacillus thuringiensis-resistant and-susceptible Ostrinia nubilalis (Lepidoptera: Crambidae)

H Li, B Oppert, RA Higgins, F Huang, KY Zhu… - Insect biochemistry and …, 2004 - Elsevier
Proteinase activities were compared in soluble and membrane fractions of guts obtained from
larvae of Bacillus thuringiensis-resistant and -susceptible Ostrinia nubilalis. Overall, serine …

[HTML][HTML] A novel Tenebrio molitor cadherin is a functional receptor for Bacillus thuringiensis Cry3Aa toxin

…, C Oppert, MD Lorenzen, K Morris, B Oppert… - Journal of Biological …, 2009 - ASBMB
Cry toxins produced by the bacterium Bacillus thuringiensis are effective biological
insecticides. Cadherin-like proteins have been reported as functional Cry1A toxin receptors in …

Different Mechanisms of Resistance toBacillus thuringiensis Toxins in the Indianmeal Moth

S Herrero, B Oppert, J Ferré - Applied and environmental …, 2001 - Am Soc Microbiol
Susceptibility to protoxin and toxin forms of Cry1Ab and the binding of 125 I-labeled Cry1Ab
and Cry1Ac has been examined in three Plodia interpunctella colonies, one susceptible (…

Prospecting for cellulolytic activity in insect digestive fluids

C Oppert, WE Klingeman, JD Willis, B Oppert… - … and Physiology Part B …, 2010 - Elsevier
Efficient cellulolytic enzymes are needed to degrade recalcitrant plant biomass during ethanol
purification and make lignocellulosic biofuels a cost-effective alternative to fossil fuels. …