Recent single-molecule enzymology measurements with improved statistics have demonstrated
that a single enzyme molecule exhibits large temporal fluctuations of the turnover rate …

Introduction In vivo imaging provides a window into the spatially complex, rapidly evolving
physiology of the cell that structural imaging alone cannot. However, observing this …

Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence
microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes …

Enzymes are biological catalysts vital to life processes and have attracted century-long
investigation. The classic Michaelis-Menten mechanism provides a highly satisfactory description …

Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale
precision on diffraction-limited instead of specialized super-resolution microscopes. ExM works …

Small-molecule fluorophores are important tools for advanced imaging experiments. We
previously reported a general method to improve small, cell-permeable fluorophores which …

This paper summarizes our present theoretical understanding of single-molecule kinetics
associated with the Michaelis−Menten mechanism of enzymatic reactions. Single-molecule …

Although messenger RNA (mRNA) translation is a fundamental biological process, it has
never been imaged in real time in vivo with single-molecule precision. To achieve this, we …

The RelA-mediated stringent response is at the heart of bacterial adaptation to starvation
and stress, playing a major role in the bacterial cell cycle and virulence. RelA integrates …

10.7554/eLife.13617.001 Protein clustering is a hallmark of genome regulation in mammalian
cells. However, the dynamic molecular processes involved make it difficult to correlate …