▪ Abstract Eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that mediates
recruitment of ribosomes to mRNA. This event is the rate-limiting step for translation under …

Regulation of protein synthesis in eukaryotes plays a critical role in development, differentiation,
cell cycle progression, cell growth, and apoptosis (Mathews et al. 2000). Translational …

Affinity purification coupled with mass spectrometry (AP-MS) is a widely used approach for
the identification of protein-protein interactions. However, for any given protein of interest, …

The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits
to the 5′ end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5′ …

A broad range of mass spectrometers are used in mass spectrometry (MS)-based
proteomics research. Each type of instrument possesses a unique design, data system and …

INTRODUCTION Genetic interactions occur when mutations in two or more genes combine
to generate an unexpected phenotype. An extreme negative or synthetic lethal genetic …

In most instances, translation is regulated at the initiation phase, when a ribosome is recruited
to the 5′ end of an mRNA. The eIF4E-binding proteins (4E-BPs) interdict translation …

The versatile combination of affinity purification and mass spectrometry (AP–MS) has recently
been applied to the detailed characterization of many protein complexes and large protein-…

Rapamycin potently inhibits downstream signaling from the target of rapamycin (TOR) proteins.
These evolutionarily conserved protein kinases coordinate the balance between protein …

Mass spectrometry–based quantitative proteomics has become an important component of
biological and clinical research. Although such analyses typically assume that a protein's …