Biosynthesis of a broad-spectrum nicotianamine-like metallophore in Staphylococcus aureus
G Ghssein, C Brutesco, L Ouerdane, C Fojcik, A Izaute… - Science, 2016 - science.org
Metal acquisition is a vital microbial process in metal-scarce environments, such as inside a
host. Using metabolomic exploration, targeted mutagenesis, and biochemical analysis, we …
host. Using metabolomic exploration, targeted mutagenesis, and biochemical analysis, we …
Ionising radiation induces the expression of PARP-1 and PARP-2 genes in Arabidopsis
G Doucet-Chabeaud, C Godon, C Brutesco… - Molecular Genetics and …, 2001 - Springer
By screening for Arabidopsis genes activated by ionising radiation (IR)-induced DNA damage,
we have isolated a cDNA hybridising with a 3.2-kb mRNA that accumulates rapidly and …
we have isolated a cDNA hybridising with a 3.2-kb mRNA that accumulates rapidly and …
[HTML][HTML] Pseudomonas aeruginosa zinc uptake in chelating environment is primarily mediated by the metallophore pseudopaline
…, L Ouerdane, C Brutesco, G Ghssein, C Hajjar… - Scientific reports, 2017 - nature.com
… Using the accurate mass and a molecular formula finder software we proposed the C 15
H 20 N 4 O 8 empiric formula for the ligand in complex with nickel or zinc (Fig. 3). This ligand …
H 20 N 4 O 8 empiric formula for the ligand in complex with nickel or zinc (Fig. 3). This ligand …
[HTML][HTML] Lipid Phosphate Phosphatases in Arabidopsis: Regulation of the AtLPP1 gene in response to stress
… The function of DG remains unclear because a protein kinase C, the downstream effector
target of DG in mammalian cells, has not been isolated from plants. So far, plant lipid …
target of DG in mammalian cells, has not been isolated from plants. So far, plant lipid …
[HTML][HTML] Single-step production of a recyclable nanobiocatalyst for organophosphate pesticides biodegradation using functionalized bacterial magnetosomes
…, R Pardoux, G Adryanczyk, D Garcia, C Brutesco… - PloS one, 2011 - journals.plos.org
… Magnetosomes still magnetically retained on the column are stored at 4C overnight and
the entire set of experiments is repeated the next day. The hydrolysis activities are plotted for …
the entire set of experiments is repeated the next day. The hydrolysis activities are plotted for …
Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana
JG Berrin, O Pierrugues, C Brutesco, B Alonso… - Molecular Genetics and …, 2005 - Springer
… For in vitro experiments, surface-sterilised seeds were sown on Murashige and Skoog
medium (Sigma, MO, USA), kept in the dark for 24 h at 4C and then grown under the light/dark …
medium (Sigma, MO, USA), kept in the dark for 24 h at 4C and then grown under the light/dark …
Bacterial host and reporter gene optimization for genetically encoded whole cell biosensors
C Brutesco, S Prévéral, C Escoffier… - … Science and Pollution …, 2017 - Springer
… We measured the bioluminescence emission after 45 min of incubation at 30 C in 2-ml …
from TECAN, the kinetics were measured at 30 C under regular shaking in sterilized Greiner 96-…
from TECAN, the kinetics were measured at 30 C under regular shaking in sterilized Greiner 96-…
Involvement of the Pseudomonas aeruginosa MexAB–OprM efflux pump in the secretion of the metallophore pseudopaline
…, L Ouerdane, C Laffont, C Brutesco… - Molecular …, 2021 - Wiley Online Library
… The cycling parameters of the qRT-PCR were 98C for 2 min, followed by 45 cycles of 98C
for 5 s, 60C for 10 s. A final melting curve from 65C to 95C was added to check the specificity …
for 5 s, 60C for 10 s. A final melting curve from 65C to 95C was added to check the specificity …
A bioluminescent arsenite biosensor designed for inline water analyzer
…, C Brutesco, ECT Descamps, C Escoffier… - … Science and Pollution …, 2017 - Springer
… Evaluation of the shelf life of these lyophilized biosensors kept at 4 C over a year evidenced
that about 40 % of the lyophilized cells can be revived in such storage conditions. The …
that about 40 % of the lyophilized cells can be revived in such storage conditions. The …
[HTML][HTML] Modulation of Metabolism and Switching to Biofilm Prevail over Exopolysaccharide Production in the Response of Rhizobium alamii to Cadmium
… Plates were incubated at 30C for 96 h. … alamii wt and the MSΔGT mutant, were examined
after 3 days and 5 days of incubation at 30C. The constitutive expression of GFP allowed the …
after 3 days and 5 days of incubation at 30C. The constitutive expression of GFP allowed the …