[HTML][HTML] In Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation

M Bouvet, C Debarnot, I Imbert, B Selisko… - PLoS …, 2010 - journals.plos.org
… Nsp14 was expressed as a fusion protein with an intein tag at its C-terminus. The nsp14-intein
product was bound to a chitin affinity column and the untagged protein was eluted after …

[HTML][HTML] Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2′-O-methyltransferase nsp10/nsp16 complex

E Decroly, C Debarnot, F Ferron, M Bouvet… - PLoS …, 2011 - journals.plos.org
… The missing helix B and helix C are indicated by dashed ovals. The approximate general …
patch A, B, C, D, and E are rendered as sticks as above in transparency into nsp10. C) A plot of …

[PDF][PDF] Nanoscale architecture of the axon initial segment reveals an organized and robust scaffold

C Leterrier, J Potier, G Caillol, C Debarnot, FR Boroni… - Cell reports, 2015 - cell.com
… Using multicolor 3D-STORM, we resolve the nanoscale organization of ankyrin G: its amino
terminus associates with the submembrane lattice, whereas the C terminus radially extends (…

[HTML][HTML] Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases

B Selisko, H Dutartre, JC Guillemot, C Debarnot… - Virology, 2006 - Elsevier
… the catalytic residues of motifs A to C within the palm subdomain as well as … The C-terminus
of flavivirus NS5 does not contain a … 2C shows all proteins used in this study after a two-step …

Why are pharmacokinetic data summarized by arithmetic means?

SA Julious, CAM Debarnot - Journal of biopharmaceutical statistics, 2000 - Taylor & Francis
… For C max we have a mean of 14 and a SD of 22, giving a normal range of −30 to 58,
thus the normal range has subjects with a negative C max . This is patently impossible, and …

Myogenic enhancers regulate expression of the facioscapulohumeral muscular dystrophy-associated DUX4 gene

CL Himeda, C Debarnot, S Homma… - … and cellular biology, 2014 - Am Soc Microbiol
… SYBR green quantitative PCR assays were performed for 40 cycles of 94C for 15 s, 55C for
30 s, and 72C for 30 s. PCR products were analyzed on a 1.5% agarose gel to verify correct …

[HTML][HTML] Molecular mapping of the RNA Cap 2′-O-methyltransferase activation interface between severe acute respiratory syndrome coronavirus nsp10 and nsp16

…, A Hermant, JC Guillemot, C Debarnot… - Journal of Biological …, 2010 - ASBMB
Several protein-protein interactions within the SARS-CoV proteome have been identified,
one of them being between non-structural proteins nsp10 and nsp16. In this work, we have …

Design, synthesis, and biological evaluation of tetrahydroisoquinoline based hydroxamate derivatives as HDAC 6 inhibitors for cancer therapy

…, BVS Kumar, M Schweipert, C Debarnot… - Journal of Molecular …, 2023 - Elsevier
… The structures of the synthesized compounds are confirmed using 1 H NMR, 13 C NMR,
LC-MS, and elemental analysis. The characterized compounds are screened for their inhibition …

Patient-derived antibodies reveal the subcellular distribution and heterogeneous interactome of LGI1

…, F Youssouf, L El Far, C Lévêque, C Debarnot… - Brain, 2022 - academic.oup.com
Autoantibodies against leucine-rich glioma-inactivated 1 (LGI1) occur in patients with
encephalitis who present with frequent focal seizures and a pattern of amnesia consistent with …

Substrate binding mode and catalytic mechanism of human heparan sulfate d-glucuronyl C5 epimerase

C Debarnot, YR Monneau… - Proceedings of the …, 2019 - National Acad Sciences
… Real-time monitoring of the enzymatic activity of this recombinant hGlce by NMR spectroscopy
using a 13 C-labeled N-sulfated heparosan substrate revealed efficient conversion of …