INTRODUCTION DNA binding transcription factors (TFs) are quintessential regulators of
eukaryotic gene expression. Early studies of TFs revealed their well-structured DNA binding …

Transcription is reported to be spatially compartmentalized in nuclear transcription factories
with clusters of RNA polymerase II (Pol II). However, little is known about when these foci …

… Samples were then incubated for 1 h on ice (4 C), at room temperature (22 C), or in an
incubator at 37 C or 45 C before microscopy analysis. Labeled (without MBP tag) and unlabeled (…

… We found that c-Myc is a global explorer of the nucleus. In contrast, the positive transcription
elongation factor P-TEFb is a local explorer that oversamples its environment. Consequently…

10.7554/eLife.47098.001 RNA Polymerase II (Pol II) and transcription factors form concentrated
hubs in cells via multivalent protein-protein interactions, often mediated by proteins with …

… In each measured nucleus, we correlated k Obs with the concentration c of RevTetR-GFP,
measured with two-photon fluorescence correlation spectroscopy (FCS) 46 (Fig. 4b inset and …

Gene activation by mammalian transcription factors (TFs) requires multivalent interactions of
their low-complexity domains (LCDs), but how such interactions regulate transcription …

The SAGA complex is a regulatory hub involved in gene regulation, chromatin modification,
DNA damage repair and signaling. While structures of yeast SAGA (ySAGA) have been …

… protocols: 1) 75C for 30 min, 2) 95C for 5 min, followed by 75C for 30 min, 3) 37C for 30
min in the presence of 0.4 mg/mL proteinase K, followed by 95C for 5 min and 75C for 30 min. …

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of
coronavirus disease 2019 (COVID-19), has emerged as a major global health threat. The COVID…