… C, 1 min at 55C, and 1 min at 72C. To amplify the highly GCrich exon 1, we raised the annealing
temperature to 68C … cycles of the following steps: 94C for 20 s, 68C for 8 min, followed …

… PCR (30 cycles with annealing temperature starting at 65 C and decreasing 0.5 C per cycle,
followed by 15 cycles with an annealing temperature of 65 C), using primers with M13 tails (…

To elucidate the genetic architecture of amyotrophic lateral sclerosis (ALS) and find
associated loci, we assembled a custom imputation reference panel from whole-genome-sequenced …

… Time and temperature conditions for one cycle were as follows: heat-denaturation was at 95
C for 2 min; primer-annealing was at 52 C for 2 min; polymerase-extension was at 72 C for 2 …

Exome sequencing is an effective strategy for identifying human disease genes. However,
this methodology is difficult in late-onset diseases where limited availability of DNA from …

… C, there was no loss of infectivity [105]. Another study confirms the viral stability at 4 C, and
also at 20 C and 37 C … 90, 60 and 30 min exposure at 56 C, 67 C and 75 C, respectively [106]. …

… Except where indicated, we scored growth after 3 d incubation at 28 C. YPD and YPG, YEP
plates containing 2… Similar results were obtained after incubation at 37 C (data not shown). (…

… (c) Induction of caspase 3 activity by staurosporine in FRDA fibroblasts versus controls.
Specific activities for caspase 3 were 1.50 ± 0.24 (2x SEM) and 0.96 ± 0.19 pmol of p-nitroaniline/…

Different point mutations of the mitochondrial genome, which all affect the ability of mitochondria
to translate their own genes and lead to partial defects of mtDNA-dependent respiratory …

To identify genetic factors contributing to amyotrophic lateral sclerosis (ALS), we conducted
whole-exome analyses of 1,022 index familial ALS (FALS) cases and 7,315 controls. In a …