… C for 1 min, then 18 cycles of 95 C for 50 s, 60 C for 50 s and 68 C for 1 min per kilobase of
plasmid length, then 68 C for 7 min and 4 C hold. Dpn1 (1 μl) was added to the PCR reaction …

… All C t and ΔC t values are listed in the Source Data files. C t values for the reference
genes ranged between 12 and 23 except for Rn18s, which ranged between 6 and 8. …

Understanding human embryology has historically relied on comparative approaches using
mammalian model organisms. With the advent of low-input methods to investigate genetic …

… b, c, Quantification of YAP1 (b) and GATA3 (c) normalized fluorescence intensity in outer
cells of embryos shown in a (n = 243 cells for YAP1 from 28 embryos, n = 191 cells for GATA3 …

… C for 3 min, (2) instant cooling from 95 C to 80 C, (3) holding at 80 C for 2 min, (4) first cooling
ramp from 80 C to 65 C at −0.3 C … C to 37 C at −0.5 C min −1 , (6) holding at 37 C for 24 h. …

… BRD7880 — aurora kinases B/C. Our first example does not discuss the targeting of a novel
… 3) as a highly selective inhibitor of aurora kinases B and C 119 . In this study, pools of DNA-…

This established textbook provides an accessible but comprehensive introduction to the
quantum nature of light and its interaction with matter. The field of quantum optics is covered …

… overnight at 4 C and subsequently dehydrated in methanol and stored at −20 C until needed.
In … Embryos were pre-incubated with hybridization buffer at 70 C for 3 h and then incubated …

… 0.5% sucrose thawing medium and incubated for 5 min at 37 C, followed by 0.2% sucrose
thawing medium for 10 min at 37 C. The embryos were then washed through seven drops of …

Originally published in 1979. In this forcefully argued book, Milton Santos shows that
contemporary explanations of urbanization and spatial organization in underdeveloped countries …