… Fu, DE Cane, C. Khosla, unpublished results). The reasons for this reduction in yield are
not clear. Developing strategies to overcome these deleterious effects on chain transfer …

… 6 -amino group of Lys has been oxidized to the nitro group and a cyclopropane has formed
by a CC bond-forming step between C 4 and C 6 . Two possible variations of the ring …

… coexpressed with the act CLF (K Burson, C Khosla, unpublished results). Together these
results … between the C-9 ketone and the C-14 methylene of the poly-β-ketone backbone (84). …

Celiac Sprue, a widely prevalent autoimmune disease of the small intestine, is induced in
genetically susceptible individuals by exposure to dietary gluten. A 33-mer peptide was …

… Induction at 37C or 25” C yielded predominantly insoluble 0195 protein (21.8 kDa), that
could be soluhilized in 8 M uiea, purified by Q-Sepharose chromatography under denaturing …

… M9 minimal media cultures of transformed cells were grown at 37C to mid-log phase,
followed by induction at 22C with 0.5 liter mM IPTG, arabinose (2.5 g/liter), and [1- 14 …

… Model building and correction were performed by using σ A -weighted F o – F c and 2F o
– F c electron density … This paper is dedicated to the memory of Don C. Wiley. We thank Dr. …

… A given ketoreductase can recognize and reduce polyketide chains of different length; this
ketoreduction always occurs at the C-9 position. The regiospecificity of the first cyclization of …

… CD4 + T cells (C) in the mLN was evaluated by means of flow cytometry. (D to G) OT-II +
CD45.1 + CD4 + T cells were transferred into WT CD45.2 + or IFNAR −/− CD45.2 + mice. …

… ends with the cyclization by the TE domain in EpoF to yield either epothilones C and D or
the hypothetical molecule containing the OH group at C-13. Abrevia- tions: KS, β-ketoacyl ACP …