… ratios of specific C, N and P acquisition activities converged on 1 : 1 : 1 but across ecosystems,
the ratio of C : P acquisition was inversely related to MAP and MAT while the ratio of C : N …

… Samples were initially denatured at 94 C for 3 min, then amplified using 35 cycles of 94 C
for 45 s, 50 C for 30 s and 72 C for 90 s. A final extension of 10 min at 72 C was added at the …

… Many researchers dry soil or litter to a constant mass at 60 C, but true dry mass is
determined by drying samples at 105 C, above the boiling point of water. Drying at 60 …

Gut microbial communities represent one source of human genetic and metabolic diversity.
To examine how gut microbiomes differ among human populations, here we characterize …

… held at 94C for 3 min to denature the DNA, with amplification proceeding for 35 cycles at 94
C for 45 s, 50 C for 60 s, and 72 C for 90 s; a final extension of 10 min at 72 C was added to …

DNA sequencing continues to decrease in cost with the Illumina HiSeq2000 generating up
to 600 Gb of paired-end 100 base reads in a ten-day run. Here we present a protocol for …

Soils collected across a long-term liming experiment (pH 4.0–8.3), in which variation in factors
other than pH have been minimized, were used to investigate the direct influence of pH on …

The present outbreak of lower respiratory tract infections, including respiratory distress
syndrome, is the third spillover, in only two decades, of an animal coronavirus to humans …

Soils harbor enormously diverse bacterial populations, and soil bacterial communities can
vary greatly in composition across space. However, our understanding of the specific …

Elucidating the biogeography of bacterial communities on the human body is critical for
establishing healthy baselines from which to detect differences associated with diseases. To …