[HTML][HTML] Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood

…, U Wirkner, U Krause, J Blake, C Schwager… - Experimental …, 2005 - Elsevier
… 3 mM MgCl at 30 seconds preincubation at 95C followed by 45 cycles of 5 seconds at 55C,
15 seconds at 72C, and 2 seconds at 95C. PCR products were subjected to melting curve …

Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis

…, B Sproat, J Stegemann, C Schwager… - Nucleic acids …, 1987 - academic.oup.com
A simple system has been designed enabling ultrasensitive on-line detection of fluorescently
labelled macromolecules, eg nucleic acids, proteins and peptides during electrophoretic …

Automated DNA sequencing of the human HPRT locus

…, H Voss, P Rice, A Civitello, J Stegemann, C Schwager… - Genomics, 1990 - Elsevier
The complete sequence of 57 kb of the human HPRT locus has been determined using
automated fluorescent DNA sequencing. The strategy employed increasingly directed …

Optimization of oligonucleotide-based DNA microarrays

A Relógio, C Schwager, A Richter… - Nucleic acids …, 2002 - academic.oup.com
… the coated glass slides with the spotted oligos for 4 h at 60C and 10 min at 120C. Alternative
attachment protocols, eg overnight incubation at 37C, resulted in decreased sensitivity. …

[PDF][PDF] Simultaneous targeting of TGF-β/PD-L1 synergizes with radiotherapy by reprogramming the tumor microenvironment to overcome immune evasion

…, C Zhou, G Locke, D Krunic, MG Derner, C Schwager… - Cancer cell, 2021 - cell.com
… (B) Representative transversal FDG-PET/computed tomography scans and (C) FDG-PET
measurement by … (B and C) Ex vivo BLI of lung metastases on day Ŕ1. (B) Lung images and (…

A non-radioactive automated method for DNA sequence determination

…, BS Sproat, J Stegemann, C Schwager - Journal of Biochemical …, 1986 - Elsevier
… dye-oligonucleotide conjugate was then desalted by dialysis and stored in the dark at -20C. …
Electrophoretic runs were performed at 1000-1500 V and 50C, with the dissipated power …

Transcriptional switch of dormant tumors to fast-growing angiogenic phenotype

N Almog, L Ma, R Raychowdhury, C Schwager… - Cancer research, 2009 - AACR
… and Janusz Weremowicz for help with technical issues and supplies; Lars Holmgren for
providing the antibody for angiomotin and helpful comments; Thuy Trinh and Barbara Schwager

Embryonic Stem Cell-Like Features of Testicular Carcinoma in Situ Revealed by Genome-Wide Gene Expression Profiling

…, CE Hoei-Hansen, U Wirkner, J Blake, C Schwager… - Cancer research, 2004 - AACR
C; 5 cycles of 30 s at 95C, 1 min at 45C, and 1 min at 72C; and 20 cycles of 30 s at 95C, 1 min
at 65C, 1 min at 72C, and finally 5 min at 72C… at 50C, and hybridized overnight at 50C with …

Complete DNA sequence of yeast chromosome XI

…, M Rose, T Rupp, MA Santos, C Schwager, C Sensen… - Nature, 1994 - nature.com
The complete DNA sequence of the yeast Saccharomyces cerevisiae chromosome XI has
been determined. In addition to a compact arrangement of potential protein coding sequences…

Transcriptional network governing the angiogenic switch in human pancreatic cancer

A Abdollahi, C Schwager, J Kleeff… - Proceedings of the …, 2007 - National Acad Sciences
A shift of the angiogenic balance to the proangiogenic state, termed the “angiogenic switch,”
is a hallmark of cancer progression. Here we devise a strategy for identifying genetic …