… Melting curves were recorded from 35 to 95C with a temperature gradient of 1C/min at a
fixed wavelength of 205 nm. A protein concentration between 0.1 and 0.2 mg/ml in 50 mM …

… Heat-induced unfolding was monitored by increasing the temperature from 35 C to 95 C
at a rate of 1 C/min, and recording the fluorescence intensity at 205 nm as a function of …

Uncertainties surrounding tree carbon allocation to growth are a major limitation to projections
of forest carbon sequestration and response to climate change. The prevalence and …

Nanobodies are recombinant, antigen-specific, single-domain, variable fragments of camelid
heavy chain-only antibodies. The innate supremacy of nanobodies as a renewable source …

Nanobodies are the smallest fully functional antigen-binding antibody fragments possessing
ideal properties as probes for molecular imaging. In this study we labeled the anti–human …

Forest vulnerability to drought is expected to increase under anthropogenic climate change,
and drought-induced mortality and community dynamics following drought have major …

… In conclusion, these data highlight the importance of the amino acid composition of the
nanobody's C-terminus, as it has a predominant effect on kidney retention. Moreover, we show …

… at 95C, followed by 30–35 PCR cycles (see Note 10), each cycle consisting of 60 s at 94C,
60 s at 55C, and 60 s at 72C. Include a final DNA extension step for 10 min at 72C after the …

… ) (the β-strands are named A through G for the constant domain with variable domains
having extra strands C′ and C″). An important hallmark of the immunoglobulin fold is the …

Envenoming following scorpion sting is a common emergency in many parts of the world.
Our aim was to ameliorate the current 100‐kDa horse plasma antivenom serum (PAS)‐derived …