Aggregation of alpha-synuclein (α-Syn) drives Parkinson’s disease (PD), although the initial
stages of self-assembly and structural conversion have not been directly observed inside …

Groundbreaking advances in volume electron microscopy and specimen preparation are
enabling the 3-dimensional visualisation of specimens with unprecedented detail, and driving …

Life exists in three dimensions, but until the turn of the century most electron microscopy
methods provided only 2D image data. Recently, electron microscopy techniques capable of …

Chronic stress and spatial training have been proposed to affect hippocampal structure and
function in opposite ways. Previous morphological studies that addressed structural …

Volume electron microscopy (vEM) is a group of techniques that reveal the 3D ultrastructure
of cells and tissues through continuous depths of at least 1 micrometer. A burgeoning …

Electron microscopic immunocytochemical methods were used to determine the localization,
subcellular distribution and expression of activity‐regulated cytoskeletal protein (Arc/Arg3.1…

Chronic restraint stress is known to affect the morphology and synaptic organization of the
hippocampus, predominantly within CA3 but also in CA1 and dentate gyrus. In this study, we …

Fluorescence microscopy of GFP-tagged proteins is a fundamental tool in cell biology, but
without seeing the structure of the surrounding cellular space, functional information can be …

The processes of life take place in multiple dimensions, but imaging these processes in even
three dimensions is challenging. Here, we describe a workflow for 3D correlative light and …

… Peddie and Anne Weston produced and analysed volume EM data. The Zooniverse
volunteers contributed many segmentations, without which this project would not have been …