An EDS1-SAG101 Complex Is Essential for TNL-Mediated Immunity in Nicotiana benthamiana

J Gantner, J Ordon, C Kretschmer, R Guerois… - The Plant …, 2019 - academic.oup.com
Heterodimeric complexes containing the lipase-like protein ENHANCED DISEASE
SUSCEPTIBILITY1 (EDS1) are regarded as central regulators of plant innate immunity. In this context…

EDS1 complexes are not required for PRR responses and execute TNL‐ETI from the nucleus in Nicotiana benthamiana

…, SL Villanueva, S Zantop, C Kretschmer… - New …, 2022 - Wiley Online Library
Heterodimeric complexes incorporating the lipase‐like proteins EDS1 with PAD4 or SAG101
are central hubs in plant innate immunity. EDS1 functions encompass signal relay from TIR …

Highly efficient multiplex editing: one‐shot generation of 8× Nicotiana benthamiana and 12× Arabidopsis mutants

…, JL Erickson, R Herr, F Ferik, C Kretschmer… - The Plant …, 2021 - Wiley Online Library
Genome editing by RNA‐guided nucleases, such as SpCas9, has been used in numerous
different plant species. However, to what extent multiple independent loci can be targeted …

[HTML][HTML] Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system

J Gantner, J Ordon, T Ilse, C Kretschmer, R Gruetzner… - PloS one, 2018 - journals.plos.org
Standardized DNA assembly strategies facilitate the generation of multigene constructs from
collections of building blocks in plant synthetic biology. A common syntax for hierarchical …

Optimized Cas9 expression systems for highly efficient Arabidopsis genome editing facilitate isolation of complex alleles in a single generation

J Ordon, M Bressan, C Kretschmer, L Dall'Osto… - Functional & integrative …, 2020 - Springer
Genetic resources for the model plant Arabidopsis comprise mutant lines defective in almost
any single gene in reference accession Columbia. However, gene redundancy and/or close …

Targeting specificity of nuclear-encoded organelle proteins with a self-assembling split-fluorescent protein toolkit

M Sharma, C Kretschmer, C Lampe… - Journal of Cell …, 2019 - journals.biologists.com
A large number of nuclear-encoded proteins are targeted to the organelles of endosymbiotic
origin, namely mitochondria and plastids. To determine the targeting specificity of these …

Receptor-like cytoplasmic kinases belonging to different subfamilies mediate immune responses downstream of the Cf-4 resistance protein in Nicotiana benthamiana

WRH Huang, C Braam, C Kretschmer, H Liu, F Ferik… - BioRxiv, 2023 - biorxiv.org
Cell-surface receptors, which are either receptor-like proteins (RLPs) or receptor-like
kinases (RLKs), form the first layer of the plant innate immune system. The LRR-RLKs …

Differential requirement for the EDS1 catalytic triad in A. thaliana and N. benthamiana

…, K Barthel, L Eschen-Lippold, S Zantop, C Kretschmer… - bioRxiv, 2021 - biorxiv.org
Heterodimeric complexes incorporating the lipase-like proteins EDS1 with PAD4 or SAG101
are central hubs in plant innate immunity. EDS1 functions encompass signal relay from TIR …

[HTML][HTML] Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two

J Ordon, N Kiel, D Becker, C Kretschmer… - Plant Methods, 2023 - Springer
Background In plant genome editing, RNA-guided nucleases such as Cas9 from Streptococcus
pyogenes (SpCas9) predominantly induce small insertions or deletions at target sites. …

[PDF][PDF] One-shot generation of duodecuple (12x) mutant Arabidopsis: Highly efficient routine editing in model species

…, J Ordon, JL Erickson, J Gantner, R Herr, C Kretschmer… - bioRxiv, 2020 - researchgate.net
… Erickson1, Johannes Gantner1, Rosalie Herr1, Carola Kretschmer1, Thomas Berner2,
Jens Keilwagen2, Sylvestre Marillonnet3, Johannes Stuttmann1* … Gantner, J, Ordon, J …