Cancer is a disease of the genome caused by oncogene activation and tumor suppressor
gene inhibition. Deep sequencing studies including large consortia such as TCGA and ICGC …

Hormone-gated nuclear receptors (NRs) are conserved transcriptional regulators of metabolism,
reproduction, and homeostasis. Here we show that C. elegans NHR-8 NR, a homolog …

Morphogen gradients specify cell fates during development, with a classic example being
the bone morphogenetic protein (BMP) gradient's conserved role in embryonic dorsal-ventral …

The Hunchback (Hb) transcription factor is crucial for anterior-posterior patterning of the
Drosophila embryo. The maternal hb mRNA acts as a paradigm for translational regulation due …

CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome
modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to …

Visualizing transcription live in Drosophila is providing important new insights into the
spatiotemporal regulation of transcription. Here, we describe a protocol to visualize and quantitate …

Motivation The MS2-MCP (MS2 coat protein) live imaging system allows for visualization of
transcription dynamics through the introduction of hairpin stem-loop sequences into a gene. …

Significant regulation of gene expression is mediated at the translation level. Here, we describe
protocols for imaging and analysis of translation at single mRNA resolution in both fixed …

It has become clear that the onset of widowhood, perhaps more than any other event of the
aging process, involves a dramatic change in the family status of older persons. Because …

Probing endogenous protein localization and function in vivo remains challenging due to
laborious gene targeting and monofunctional alleles. Here, we develop a multifunctional, …