Bacterial pathogens frequently use protein secretion to mediate interactions with their hosts.
Here we found that a virulence locus (HSI-I) of Pseudomonas aeruginosa encodes a protein …

DNA methylation is a key epigenetic modification involved in regulating gene expression
and maintaining genomic integrity. Here we inactivated all three catalytically active DNA …

Differentiation of human embryonic stem cells (hESCs) provides a unique opportunity to
study the regulatory mechanisms that facilitate cellular transitions in a human context. To that …

Secreted proteins are crucial to the arsenal of bacterial pathogens. Although optimal activity
of these proteins is likely to require precise regulation of release, the signalling events that …

In diseased organs, stress-activated signalling cascades alter chromatin, thereby triggering
maladaptive cell state transitions. Fibroblast activation is a common stress response in …

Induced pluripotency is a promising avenue for disease modeling and therapy, but the
molecular principles underlying this process, particularly in human cells, remain poorly …

Organogenesis involves integration of diverse cell types; dysregulation of cell-type-specific
gene networks results in birth defects, which affect 5% of live births. Congenital heart defects …

Abstract Models derived from human pluripotent stem cells that accurately recapitulate
neural development in vitro and allow for the generation of specific neuronal subtypes are of …

Research on human pluripotent stem cells has been hampered by the lack of a standardized,
quantitative, scalable assay of pluripotency. We previously described an assay called …

Complex genetic mechanisms are thought to underlie many human diseases, yet experimental
proof of this model has been elusive. Here, we show that a human cardiac anomaly can …