User profiles for Cem Kuscu
 | CEM KUSCUAssistant Professor, University of Tennessee Health Science Center Verified email at uthsc.edu Cited by 3771 |
Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease
RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic
and clinical research, but the determinants of targeting specificity and the extent of off-target …
and clinical research, but the determinants of targeting specificity and the extent of off-target …
CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations
CRISPR–Cas9-induced DNA damage may have deleterious effects at high-copy-number
genomic regions. Here, we use CRISPR base editors to knock out genes by changing single …
genomic regions. Here, we use CRISPR base editors to knock out genes by changing single …
Deletion of Asxl1 results in myelodysplasia and severe developmental defects in vivo
Somatic Addition of Sex Combs Like 1 (ASXL1) mutations occur in 10–30% of patients with
myeloid malignancies, most commonly in myelodysplastic syndromes (MDSs), and are …
myeloid malignancies, most commonly in myelodysplastic syndromes (MDSs), and are …
[HTML][HTML] Inhibition of matrix metalloproteinase 14 (MMP-14)-mediated cancer cell migration
Matrix metalloproteinases (MMPs) have been shown to be key players in both extracellular
matrix remodeling and cell migration during cancer metastasis. MMP-14, a membrane-…
matrix remodeling and cell migration during cancer metastasis. MMP-14, a membrane-…
Cas9-chromatin binding information enables more accurate CRISPR off-target prediction
The CRISPR system has become a powerful biological tool with a wide range of
applications. However, improving targeting specificity and accurately predicting potential off-targets …
applications. However, improving targeting specificity and accurately predicting potential off-targets …
[HTML][HTML] Live cell imaging of low-and non-repetitive chromosome loci using CRISPR-Cas9
Imaging chromatin dynamics is crucial to understand genome organization and its role in
transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been …
transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been …
miR-181a-5p inhibits cancer cell migration and angiogenesis via downregulation of matrix metalloproteinase-14
Y Li, C Kuscu, A Banach, Q Zhang, A Pulkoski-Gross… - Cancer research, 2015 - AACR
Upregulation of matrix metalloproteinase MMP-14 (MT1-MMP) is associated with poor prognosis
in cancer patients, but it is unclear how MMP-14 becomes elevated in tumors. Here, we …
in cancer patients, but it is unclear how MMP-14 becomes elevated in tumors. Here, we …
[HTML][HTML] Role of matrix metalloproteinase-9 dimers in cell migration: design of inhibitory peptides
Non-proteolytic activities of matrix metalloproteinases (MMPs) have recently been shown to
impact cell migration, but the precise mechanism remains to be understood. We previously …
impact cell migration, but the precise mechanism remains to be understood. We previously …
Small-molecule anticancer compounds selectively target the hemopexin domain of matrix metalloproteinase-9
Lack of target specificity by existing matrix metalloproteinase (MMP) inhibitors has hindered
antimetastatic cancer drug discovery. Inhibitors that bind to noncatalytic sites of MMPs and …
antimetastatic cancer drug discovery. Inhibitors that bind to noncatalytic sites of MMPs and …
Unraveling the role of KIAA1199, a novel endoplasmic reticulum protein, in cancer cell migration
NA Evensen, C Kuscu, HL Nguyen… - Journal of the …, 2013 - academic.oup.com
Background Cell migration is a critical determinant of cancer metastasis, and a better
understanding of the genes involved will lead to the identification of novel targets aimed at …
understanding of the genes involved will lead to the identification of novel targets aimed at …