The segregation of many bacterial chromosomes is dependent on the interactions of ParB
proteins with centromere-like DNA sequences called parS that are located close to the origin …

Faithful segregation of bacterial chromosomes relies on the ParABS partitioning system and
the SMC complex. In this work, we used single-molecule techniques to investigate the role …

10.7554/eLife.28086.001 The ParB protein forms DNA bridging interactions around parS to
condense DNA and earmark the bacterial chromosome for segregation. The molecular …

A-tracts are A:T rich DNA sequences that exhibit unique structural and mechanical properties
associated with several functions in vivo. The crystallographic structure of A-tracts has …

Many bacterial plasmids replicate by an asymmetric rolling-circle mechanism that requires
sequence-specific recognition for initiation, nicking of one of the template DNA strands and …

10.7554/eLife.43812.001 Bacillus subtilis ParB forms multimeric networks involving non-specific
DNA binding leading to DNA condensation. Previously, we found that an excess of the …

Combining single-molecule techniques with fluorescence microscopy has attracted much
interest because it allows the correlation of mechanical measurements with directly visualized …

Many bacterial species are helical in shape, including the widespread pathogen H. pylori.
Motivated by recent experiments on H. pylori showing that cell wall synthesis is not uniform [JA …

The rolling-circle replication is the most common mechanism for the replication of small
plasmids carrying antibiotic resistance genes in Gram-positive bacteria. It is initiated by the …

Take a drinking straw and bend it from its ends. After sufficient bending, the tube buckles
forming a kink, where the curvature is localized in a very small area. This instability, known …