Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread to nearly every
corner of the globe, causing societal instability. The resultant coronavirus disease 2019 (…

Decades of work have aimed to genetically reprogram T cells for therapeutic purposes 1 , 2
using recombinant viral vectors, which do not target transgenes to specific genomic sites 3 , …

Background Conditional knockout mice and transgenic mice expressing recombinases,
reporters, and inducible transcriptional activators are key for many genetic studies and comprise …

CRISPR/Cas9-based genome editing can easily generate knockout mouse models by disrupting
the gene sequence, but its efficiency for creating models that require either insertion of …

The availability of techniques to create desired genetic mutations has enabled the laboratory
mouse as an extensively used model organism in biomedical research including human …

Genome editing using the CRISPR/Cas9 system requires the presence of guide RNAs bound
to the Cas9 endonuclease as a ribonucleoprotein (RNP) complex in cells, which cleaves …

We present a robust method called improved-Genome editing via Oviductal Nucleic Acids
Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7 embryos via in situ …

Background Alzheimer’s disease (AD) is a progressive neurodegenerative disorder
characterized by pathological deposition of misfolded self-protein amyloid beta (Aβ) which in kind …

Knockdown mouse models, where gene dosages can be modulated, provide valuable
insights into gene function. Typically, such models are generated by embryonic stem (ES) cell-…

Over the past decade, new methods and procedures have been developed to generate
genetically engineered mouse models of human disease. This At a Glance article highlights …