Embryonic stem (ES) cells can undergo many aspects of mammalian embryogenesis in vitro
1 , 2 , 3 , 4 – 5 , but their developmental potential is substantially extended by interactions …

INTRODUCTION During preimplantation development, the establishment of apicobasal cell
polarity is key for the transition from totipotency to pluripotency, which induces cell …

Droplet microfluidic methods have massively increased the throughput of single-cell
sequencing campaigns. The benefit of scale-up is, however, accompanied by increased …

Human embryogenesis entails complex signalling interactions between embryonic and
extra-embryonic cells. However, how extra-embryonic cells direct morphogenesis within the …

The metabotropic glutamate receptor 5 (mGlu5) and N -methyl- d -aspartate (NMDA) receptor
are critical for processes underlying synaptic plasticity, such as long-term potentiation. …

Stem cell-based mammalian embryo models facilitate the discovery of developmental
mechanisms because they are more amenable to genetic and epigenetic perturbations than …

In this chapter, we detail the experimental protocol leading to the generation of stem cell-based
mouse embryo-like structures termed “ETiX-embryoids.” ETiX-embryoids are formed from …

Embryo-like structures generated from stem cells can achieve varying developmental
milestones, but none have been shown to progress through gastrulation, neurulation, and …

The establishment of cell polarity de novo in the early mammalian embryo triggers the transition
from totipotency to differentiation to generate embryonic and extra-embryonic lineages. …

Mammalian embryogenesis begins with the fusion of an oocyte and a sperm to create the
first cell of the newly developing embryo called the zygote. The mechanisms by which this …