User profiles for Chia-Feng Tsai
 | Chia-Feng TsaiScorpion Therapeutics, PNNL, Kyoto University, Academia Sinica Verified email at ntu.edu.tw Cited by 3259 |
[HTML][HTML] Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics
Large numbers of cells are generally required for quantitative global proteome profiling due
to surface adsorption losses associated with sample processing. Such bulk measurement …
to surface adsorption losses associated with sample processing. Such bulk measurement …
High-throughput single cell proteomics enabled by multiplex isobaric labeling in a nanodroplet sample preparation platform
Effective extension of mass spectrometry-based proteomics to single cells remains challenging.
Herein we combined microfluidic nanodroplet technology with tandem mass tag (TMT) …
Herein we combined microfluidic nanodroplet technology with tandem mass tag (TMT) …
[HTML][HTML] An improved boosting to amplify signal with isobaric labeling (iBASIL) strategy for precise quantitative single-cell proteomics
Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations
of antibody-based immunoassays for antibody-independent, comprehensive, and …
of antibody-based immunoassays for antibody-independent, comprehensive, and …
[HTML][HTML] High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip
Global quantification of protein abundances in single cells could provide direct information
on cellular phenotypes and complement transcriptomics measurements. However, single-cell …
on cellular phenotypes and complement transcriptomics measurements. However, single-cell …
Automated coupling of nanodroplet sample preparation with liquid chromatography–mass spectrometry for high-throughput single-cell proteomics
Single-cell proteomics can provide critical biological insight into the cellular heterogeneity
that is masked by bulk-scale analysis. We have developed a nanoPOTS (nanodroplet …
that is masked by bulk-scale analysis. We have developed a nanoPOTS (nanodroplet …
[PDF][PDF] Proteogenomic and metabolomic characterization of human glioblastoma
Glioblastoma (GBM) is the most aggressive nervous system cancer. Understanding its
molecular pathogenesis is crucial to improving diagnosis and treatment. Integrated analysis of …
molecular pathogenesis is crucial to improving diagnosis and treatment. Integrated analysis of …
[PDF][PDF] Proteogenomic characterization of endometrial carcinoma
We undertook a comprehensive proteogenomic characterization of 95 prospectively collected
endometrial carcinomas, comprising 83 endometrioid and 12 serous tumors. This analysis …
endometrial carcinomas, comprising 83 endometrioid and 12 serous tumors. This analysis …
[HTML][HTML] Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics
Our ability to model the dynamics of signal transduction networks will depend on accurate
methods to quantify levels of protein phosphorylation on a global scale. Here we describe a …
methods to quantify levels of protein phosphorylation on a global scale. Here we describe a …
[PDF][PDF] Three-dimensional feature matching improves coverage for single-cell proteomics based on ion mobility filtering
Single-cell proteomics (scProteomics) promises to advance our understanding of cell functions
within complex biological systems. However, a major challenge of current methods is …
within complex biological systems. However, a major challenge of current methods is …
[HTML][HTML] A data-independent acquisition-based global phosphoproteomics system enables deep profiling
Phosphoproteomics can provide insights into cellular signaling dynamics. To achieve deep
and robust quantitative phosphoproteomics profiling for minute amounts of sample, we here …
and robust quantitative phosphoproteomics profiling for minute amounts of sample, we here …