Many chromatin features play critical roles in regulating gene expression. A complete
understanding of gene regulation will require the mapping of specific chromatin features in small …

Chemical mutagenesis has been the workhorse of traditional genetics, but it has not been
possible to determine underlying rates or distributions of mutations from phenotypic screens. …

TILLING (Targeting Induced LocalLesions in Genomes) is a general reverse-genetic
strategy that provides an allelic series of induced point mutations in genes of interest. High-…

We have adapted the mutation detection technology used in Targeting Induced Local
Lesions in Genomes (TILLING) to the discovery of polymorphisms in natural populations. The …

Background Going from a gene sequence to its function in the context of a whole organism
requires a strategy for targeting mutations, referred to as reverse genetics. Reverse genetics …

Chromatin profiling at locus resolution uncovers gene regulatory features that define cell
types and developmental trajectories, but it remains challenging to map and compare different …

Targeting-induced local lesions in genomes (TILLING) is a general strategy for identifying
induced point mutations that can be applied to almost any organism. Here, we describe the …

10.7554/eLife.01861.001 In budding yeast, a single cenH3 (Cse4) nucleosome occupies the
∼120-bp functional centromere, however conflicting structural models for the particle have …

Cleavage under targets and tagmentation (CUT&Tag) is an antibody-directed in situ chromatin
profiling strategy that is rapidly replacing immune precipitation-based methods, such as …

The structure of nucleosomes that contain the cenH3 histone variant has been controversial.
In budding yeast, a single right-handed cenH3/H4/H2A/H2B tetramer wraps the ∼80-bp …