The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference (CRISPRi),
and activation (CRISPRa) has enabled the systematic interrogation of gene function. …

Combinatorial genetic screening using CRISPR–Cas9 is a useful approach to uncover
redundant genes and to explore complex gene networks. However, current methods suffer from …

Examines the extent to which academic achievement and work habits of first and second
graders are predicted by classroom social status over the kindergarten, first-, and second-grade …

Anthropogenic disturbance is a relevant and widespread facilitator of environmental change
and there is clear evidence that it impacts natural populations. While population-level …

Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic perturbations
because they can process multiple guide RNAs expressed as a single transcript, and …

Isogenic pairs of cell lines, which differ by a single genetic modification, are powerful tools for
understanding gene function. Generating such pairs of mammalian cells, however, is labor-…

10.7554/eLife.40260.001 We find that variation in the dbt-1 gene underlies natural differences
in Caenorhabditis elegans responses to the toxin arsenic. This gene encodes the E2 …

Many chemotherapeutic drugs are differentially effective from one patient to the next.
Understanding the causes of this variability is a critical step towards the development of …

The objective of this study was to investigate whether juvenile Iberian pigs with diet-induced
nonalcoholic fatty liver disease (NAFLD), cholestasis, and gut dysbiosis would develop …

Many implementations of pooled screens in mammalian cells rely on linking an element of
interest to a barcode, with the latter subsequently quantitated by next generation sequencing. …