The CRISPR–Cas9 system is a powerful tool for genome editing, which allows the precise
modification of specific DNA sequences. Many efforts are underway to use the CRISPR–Cas9 …

Translation of the CRISPR–Cas9 system to human therapeutics holds high promise. However,
specificity remains a concern especially when modifying stem cell populations. We show …

Programmable genome integration of large, diverse DNA cargo without DNA repair of
exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We …

Originally described as a repressor of gene expression in the stationary phase of growth,
CsrA (RsmA) regulates primary and secondary metabolic pathways, biofilm formation, motility, …

Most bacterial pathogens have the remarkable ability to flourish in the external environment
and in specialized host niches. This ability requires their metabolism, physiology, and …

Sickle cell disease (SCD) is the most common serious monogenic disease with 300,000 births
annually worldwide. SCD is an autosomal recessive disease resulting from a single point …

Though AsCas12a fills a crucial gap in the current genome editing toolbox, it exhibits relatively
poor editing efficiency, restricting its overall utility. Here we isolate an engineered variant, …

Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is a destructive citrus disease
worldwide. Generating disease-resistant cultivars is the most effective, environmentally …

CsrA protein regulates important cellular processes by binding to target mRNAs and altering
their translation and/or stability. In Escherichia coli, CsrA binds to sRNAs, CsrB and CsrC, …

Csr is a conserved global regulatory system that controls expression of several hundred E
scherichia coli genes. CsrA protein represses translation of numerous genes by binding to …