Competitive coevolution between microbes and viruses has led to the diversification of
CRISPR–Cas defense systems against infectious agents. By analyzing metagenomic terabase …

Programmable RNA editing tools enable the reversible correction of mutant transcripts, reducing
the potential risk associated with permanent genetic changes associated with the use of …

Here we developed an adenine transversion base editor, AYBE, for A-to-C and A-to-T
transversion editing in mammalian cells by fusing an adenine base editor (ABE) with …

Human embryonic stem cells (ESCs) and human induced pluripotent stem cells hold great
promise for cell-based therapies and drug discovery. However, homogeneous differentiation …

CRISPR–Cas13 systems have recently been used for targeted RNA degradation in various
organisms. However, collateral degradation of bystander RNAs has limited their in vivo …

The main goal of structural reliability is to determine the failure probability of a structure by
considering the randomness of the inputs. When the failure probability is small, the simulation …

RNA knockdown in vivo carries significant potential for disease modeling and therapies.
Despite the emerging approaches of CRISPR/Cas9-mediated permanent knock out of targeted …

Structural reliability analysis aims to estimate the failure probability of a structure with respect
to the performance function. This estimation may be a difficult task when the computation of …

Approximately 10% of monogenic diseases are caused by nonsense point mutations that
generate premature termination codons (PTCs), resulting in a truncated protein and nonsense-…

Dear Editor, Mitochondrial DNA (mtDNA) is encapsulated by the organelle membrane 1
forming the barrier for the access of CRISPR-based gene-editing tools to the mtDNA. …