These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical aspects of …

After being activated by antigen, helper T lymphocytes switch from a resting state to clonal
expansion. This switch requires inactivation of the transcription factor Foxo1, a suppressor of …

The co-stimulators ICOS (inducible T cell co-stimulator) and CD28 are both important for T
follicular helper (TFH) cells, yet their individual contributions are unclear. Here, we show that …

Although T helper 17 (T H 17) cells have been acknowledged as crucial mediators of
autoimmune tissue damage, the effector cytokines responsible for their pathogenicity still remain …

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when
performing flow cytometry experiments and includes comprehensive sections describing …

The interrogation of single cells is revolutionizing biology, especially our understanding of
the immune system. Flow cytometry is still one of the most versatile and high-throughput …

Regulatory T cells (Tregs) represent a major population in the control of immune homeostasis
and autoimmunity. Here we show that Fos-like 2 (Fosl2), a TCR-induced AP1 transcription …

B cells undergo affinity maturation and class switch recombination of their immunoglobulin
receptors during a germinal center (GC) reaction, before they differentiate into long‐lived …

Allogeneic hematopoietic cell transplantation (allo-HCT) not only is an effective treatment
for several hematologic malignancies but can also result in potentially life-threatening graft-…

Repeatedly activated T helper 1 (Th1) cells present during chronic inflammation can efficiently
adapt to the inflammatory milieu, for example, by expressing the transcription factor Twist1…