As part of its remit to provide computational support to the cryo-EM community, the Collaborative
Computational Project for Electron cryo-Microscopy (CCP-EM) has produced a software …

In 2020, cryo-EM single-particle analysis achieved true atomic resolution thanks to
technological developments in hardware and software. The number of high-resolution …

This paper describes outcomes of the 2019 Cryo-EM Model Challenge. The goals were to (1)
assess the quality of models that can be produced from cryogenic electron microscopy (…

Single particle analysis has become a key structural biology technique. Experimental images
are extremely noisy, and during iterative refinement it is possible to stably incorporate …

We present LAFTER, an algorithm for de-noising single particle reconstructions from cryo-EM.
Single particle analysis entails the reconstruction of high-resolution volumes from tens of …

The use of slab-like flat specimens for electron cryo-tomography restricts the range of
viewing angles that can be used. This leads to the “missing wedge” problem, which causes …

Cryo-EM images have extremely low signal-to-noise levels because biological macromolecules
are highly radiation-sensitive, requiring low-dose imaging, and because the molecules …

The pathogenic bacteria Bordetella parapertussis and Bordetella bronchiseptica express a
lipopolysaccharide O antigen containing a polymer of 2,3-diacetamido-2,3-dideoxy-l-…

The Pi sampling method is derived from the incomplete factorial approach to macromolecular
crystallization screen design. The resulting `Pi screens' have a modular distribution of a …

This paper describes outcomes of the 2019 Cryo-EM Map-based Model Metrics Challenge
sponsored by EMDataResource ( www.emdataresource.org ). The goals of this challenge …