Technology utilizing human induced pluripotent stem cells (iPS cells) has enormous potential
to provide improved cellular models of human disease. However, variable genetic and …

Constitutive heterochromatin is responsible for genome repression of DNA enriched in
repetitive sequences, telomeres, and centromeres. During physiological and pathological …

Multiplexing strategies for large-scale proteomic analyses have become increasingly prevalent,
tandem mass tags (TMT) in particular. Here we used a large iPSC proteomic experiment …

… γ-tubulin on centrosomes were decreased (Figure 4D). This is also consistent with the
phenotypes we observed in cells depleted of PHD1 (Figure 4D). These results indicate that PHD1 …

Human disease phenotypes are driven primarily by alterations in protein expression and/or
function. To date, relatively little is known about the variability of the human proteome in …

… The plates were kept at 4 C in the dark for 2 d and then moved to a growth chamber (CU36-L5,
Percival Scientific) at 21 C under a photoperiod of 16 h light and 8 h darkness for …

… (D) Pie chart showing the overlap between quantified gene products in the proteomics
and RNA-seq datasets. (E) Scatterplot comparing the median log2 transcripts per million (TPM) …

Membraneless organelles are sites for RNA biology including small non-coding RNA (ncRNA)
mediated gene silencing. How small ncRNAs utilise phase separated environments for …

… (D) Venn diagram comparing the numbers of proteins identified in the hermaphrodite worm
… (D) Boxplot of the untransformed iBAQ values for ten control (Con) and ten heat-shocked (HS…

Data independent acquisition–mass spectrometry (DIA–MS) is becoming widely utilised for
robust and accurate quantification of samples in quantitative proteomics. Here, we describe …