User profiles for D. B. Mahat
DigBijay MahatMassachusetts Institute of Technology Verified email at mit.edu Cited by 1393 |
Base-pair-resolution genome-wide mapping of active RNA polymerases using precision nuclear run-on (PRO-seq)
We provide a protocol for precision nuclear run-on sequencing (PRO-seq) and its variant,
PRO-cap, which map the location of active RNA polymerases (PRO-seq) or transcription start …
PRO-cap, which map the location of active RNA polymerases (PRO-seq) or transcription start …
[PDF][PDF] Mammalian heat shock response and mechanisms underlying its genome-wide transcriptional regulation
The heat shock response (HSR) is critical for survival of all organisms. However, its scope,
extent, and the molecular mechanism of regulation are poorly understood. Here we show that …
extent, and the molecular mechanism of regulation are poorly understood. Here we show that …
[HTML][HTML] Transcriptional response to stress is pre-wired by promoter and enhancer architecture
… To analyze transcription of genes, we filtered RefGen database to obtain a non-redundant
list of RNA- and protein-coding genes with 500 nt or more in length (n = 23,698). The density …
list of RNA- and protein-coding genes with 500 nt or more in length (n = 23,698). The density …
Transcription factors GAF and HSF act at distinct regulatory steps to modulate stress-induced gene activation
The coordinated regulation of gene expression at the transcriptional level is fundamental to
development and homeostasis. Inducible systems are invaluable when studying …
development and homeostasis. Inducible systems are invaluable when studying …
[PDF][PDF] Transcriptional pause sites delineate stable nucleosome-associated premature polyadenylation suppressed by U1 snRNP
Regulation of RNA polymerase II (Pol II) elongation is a critical step in gene regulation. Here,
we report that U1 snRNP recognition and transcription pausing at stable nucleosomes are …
we report that U1 snRNP recognition and transcription pausing at stable nucleosomes are …
[PDF][PDF] CBP regulates recruitment and release of promoter-proximal RNA polymerase II
Transcription activation involves RNA polymerase II (Pol II) recruitment and release from the
promoter into productive elongation, but how specific chromatin regulators control these …
promoter into productive elongation, but how specific chromatin regulators control these …
[PDF][PDF] Stress-induced transcriptional memory accelerates promoter-proximal pause release and decelerates termination over mitotic divisions
A Vihervaara, DB Mahat, SV Himanen, MAH Blom… - Molecular cell, 2021 - cell.com
Heat shock instantly reprograms transcription. Whether gene and enhancer transcription
fully recover from stress and whether stress establishes a memory by provoking transcription …
fully recover from stress and whether stress establishes a memory by provoking transcription …
A rapid, sensitive, scalable method for Precision Run-On sequencing (PRO-seq)
Tracking active transcription with the nuclear run-on (NRO) assays has been instrumental in
uncovering mechanisms of gene regulation. The coupling of NROs with high-throughput …
uncovering mechanisms of gene regulation. The coupling of NROs with high-throughput …
[HTML][HTML] Use of conditioned media is critical for studies of regulation in response to rapid heat shock
… for the same period in the same conditions (Mahat et al. 2016b), and we documented the …
Highly enriched and non-redundant GO terms from the PANTHER database were selected …
Highly enriched and non-redundant GO terms from the PANTHER database were selected …
[HTML][HTML] Coordinated expression of tristetraprolin post-transcriptionally attenuates mitogenic induction of the oncogenic Ser/Thr kinase Pim-1
DB Mahat, SE Brennan-Laun, EJ Fialcowitz-White… - PLoS …, 2012 - journals.plos.org
The serine/threonine kinase Pim-1 directs selected signaling events that promote cell growth
and survival and is overexpressed in diverse human cancers. Pim-1 expression is tightly …
and survival and is overexpressed in diverse human cancers. Pim-1 expression is tightly …