We describe an isothermal, single-reaction method for assembling multiple overlapping DNA
molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA …

We report the design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma
mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its …

Here it is demonstrated that the yeast Saccharomyces cerevisiae can take up and assemble
at least 38 overlapping single-stranded oligonucleotides and a linear double-stranded …

Background The use of echocardiography in the critically ill presents specific challenges.
However, information of direct relevance to clinical management can be obtained relating to …

Background National and subnational level surveys are important for monitoring disease
burden, prioritizing resource allocation, and evaluating public health policies. As mobile phone …

In order to investigate the possibility of regional variation of ventricular structure, 25 normal
postmortem human hearts were studied by inspection of cavity shape and subepicardial fibre …

We have synthesized a 582,970–base pair Mycoplasma genitalium genome. This synthetic
genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium …

… Derek G Gibson1, … Henein MY, Gibson DG. Long axis function in disease. Heart1999;81:229–31. …
Xiao HB, Lee CH, Gibson DG. Effect of left bundle branch block on diastolic function in …

INTRODUCTION In 1984, the simplest cells capable of autonomous growth, the mycoplasmas,
were proposed as models for understanding the basic principles of life. In 1995, we …

Linda Klebe Treviño Gary R. Weaver David G. Gibson Barbara Ley Toffler en years ago, a
Business Roundtable report titled Corporate Ethics: A Prime Business Asset suggested that “…