Mitochondria are highly dynamic organelles whose functions are essential for cell viability.
Within the cell, the mitochondrial network is continuously remodeled through the balance …

The molecular components of the mitochondrial Ca 2+ uptake machinery have been only
recently identified. In the last months, in addition to the pore forming subunit and of one …

… The effective detergent to lipid ratio (R eff ) was determined by the equation R eff = (D
total - D water )/[lipid], in which D total is the total detergent concentration and D water is the …

… environment in situ may significantly affect their K d and thus any quantitative analysis in living
… An accurate measurement of the sensor K d in situ is therefore essential if any quantitative …

Calcium dynamics in astrocytes represent a fundamental signal that through gliotransmitter
release regulates synaptic plasticity and behaviour. Here we present a longitudinal study in …

… (D) ER in S2 cells under live and fixed conditions. (D′) Magnification of boxed regions in
D. … (D′) Magnification of boxed regions in D. Chemical fixation was achieved by a 5 min …

… (D) Control and RNAi lines were crossed to the ubiquitous driver tubulin-Gal4 and mitochondria
were isolated. Rate of ATP synthesis driven by malate and pyruvate was normalized to …

… (D) The bar graph reports the mean change of F/F max (±SEM; n = 16, 54, 37, and 23,
respectively) values for mitoChR2(SSFO) cells normalized to corresponding values for mitoChR2(…

… This is due to the higher K d of the probe used to monitor the [Ca 2+ ] within the ER, compared
to … (A–D) Representative traces of a typical experiment performed in fibroblasts from FAD …

… d average length of ER profiles measured on thin EM sections shown in (c), n > 100 ER
profiles. Statistical significance: unpaired two-tailed t test, ***p < 0.001. Error bars represent SEM…