Dynamic protein interactions play a significant part in many cellular processes. A technique
that shows considerable promise in elucidating such interactions is Förster resonance …

The original green fluorescent protein (GFP) was discovered back in the early 1960s when
researchers studying the bioluminescent properties of the Aequorea victoria jellyfish isolated …

… David W Piston David W Piston … Denk, W., Piston, DW and Webb, WW (1995) The
Handbook of Biological Confocal Microscopy (2nd edn) (Pawley, J., ed.), pp. 445–458, Plenum …

We have investigated properties relevant to quantitative imaging in living cells of five green
fluorescent protein (GFP) variants that have been used extensively or are potentially useful. …

… (d) Cyan and mCit channels are shown from a βTC3 cell expressing the Cerulean glucokinase
biosensor before and after insulin stimulation (100 nM, 2 min). (e,f) Spectral imaging (800-…

A clear relationship exists between visceral obesity and type 2 diabetes, whereas subcutaneous
obesity is comparatively benign. Here, we show that adipocyte-specific deletion of the …

… Arrowheads indicate expression of Bmp2 in the dorsal surface ectoderm (A,F), Bmp5 in the
most anterior neuroectoderm (C,H) and Bmp7 in the anterior foregut and notochord (D). The …

We describe a method for assaying protein interactions that offers some attractive advantages
over previous assays. This method, called bioluminescence resonance energy transfer (…

The corepressor CtBP (carboxyl-terminal binding protein) is involved in transcriptional
pathways important for development, cell cycle regulation, and transformation. We demonstrate …

… (D) Line-scan over a gold nanoparticle of 10 nm diameter at the bottom of 1.3 μm liquid (10%
PBS buffer in water) in a test sample without cells. The pixel size was 0.91 nm. See also Fig…