… (d) Selective immunoprecipitation of the methylated allele at the imprinted H19 ICR locus.
A polymorphic SacI site is used to distinguish the maternal from the paternal allele in a hybrid …

Cytosine methylation is a DNA modification generally associated with transcriptional
silencing. Factors that regulate methylation have been linked to human disease, yet how they …

In eukaryotes, all DNA-templated reactions occur in the context of chromatin. Nucleosome
packaging inherently restricts DNA accessibility for regulatory proteins but also provides an …

Methylation of cytosines can mediate epigenetic gene silencing and is the only known DNA
modification in eukaryotes. Recent efforts to map DNA methylation across mammalian …

… (D) Validation of YAP1 binding to known and novel sites, and control regions using ChIP-qPCR.
Data are representative of at least three independent experiments. Error bars indicate …

10.7554/eLife.01632.001 Post-translational modifications of proteins have emerged as a
major mechanism for regulating gene expression. However, our understanding of how histone …

To gain insight into the function of DNA methylation at cis-regulatory regions and its impact
on gene expression, we measured methylation, RNA polymerase occupancy and histone …

… d, CTCF binding in cells with or without DNA methylation. CTCF binding is globally
unchanged in cells without methylation (EStko), but increases by approximately twofold at sites …

The covalent modification of nucleosomal histones has emerged as a major determinant of
chromatin structure and gene activity. To understand the interplay between various histone …

… (D) Time course profiles for a random selection of 26 de novo methylated promoters. The
dotted red line indicates the averaged profile for all de novo methylated promoters. …